Validation of an automated enzyme immunoassay for interleukin-6 for routine clinical use

被引：22

作者：

Fraunberger, P ^{[1
]}

Pfeiffer, N ^{[1
]}

Cremer, P ^{[1
]}

Holler, E ^{[1
]}

Nagel, D ^{[1
]}

Dehart, I ^{[1
]}

Thein, M ^{[1
]}

Walli, AK ^{[1
]}

Seidel, D ^{[1
]}

机构：

[1] Univ Munich, Klinikum Grosshadern, Inst Klin Chem, D-81366 Munich, Germany

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 1998年 / 36卷 / 10期

关键词：

cytokine; Cobas Core (R); automation; inflammation; infection; sepsis; routine monitoring;

D O I：

10.1515/CCLM.1998.141

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Serum levels of Interleukin-6 (IL-6), a proinflammatory cytokine, are increased in early stages of inflammatory diseases such as infection and sepsis. Assay systems which permit its measurement within a few hours and as a single measurement have not been reported so far. We therefore evaluated a now commercially available automated method for IL-6 measurement on the Cobas Core(R) immunological analyzer (Roche Diagnostic Systems) which enables single IL-6 measurement within about 1 hour. The automated assay correlates well with an established, manual microtiter plate assay (Biosource GmbH) which uses the same antibodies and reagents (r=0.98). Accuracy of the automated method was established by adding known amounts of IL-6 international reference preparation. Recovery of the international standard was in the range of 92-104%. The automated assay had a precision of singletons below 6% and was linear up to 2800 pg/ml. This automated assay provides a suitable, convenient and time saving method for measurement of IL-6 serum levels in the routine clinical laboratory.

引用

页码：797 / 801

页数：5

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