LncRNA RPSAP52 regulates miR-423-5p/GSTM1 axis to suppress hypoxia-induced renal proximal tubular epithelial cell apoptosis

被引：0
|
作者：
Chen, Jie [1 ,2 ]
Zheng, Yu [3 ]
Li, Liping [1 ]
机构：
[1] Shantou Univ, Coll Med, Injury Prevent Res Ctr, 22 Xinling Rd, Shantou 515041, Guangdong, Peoples R China
[2] Shantou Univ, Coll Med, Affiliated Hosp 2, Dept Urol, Shantou, Peoples R China
[3] Guang Zhou Med Univ, Affiliated Hosp 2, Dept Urol, Guangzhou, Peoples R China
来源：
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | 2022年 / 128卷 / 04期
关键词：
RPSAP52; renal failure; miR-423-5p; GSTM1; human renal proximal tubular epithelial cells; KIDNEY FAILURE;
D O I：
10.1080/13813455.2020.1750657
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study aimed to investigate the roles of RPSAP52 in renal failure. Our results showed that RPSAP52 was upregulated in plasma of renal failure patients in comparison to healthy controls. Dual luciferase reporter assay showed that RPSAP52 could interact with miR-423-5p, while overexpression of RPSAP52 and miR-423-5p did not alter the expression of each other in human renal proximal tubular epithelial cells (HRPTEpCs). In addition, overexpression of RPSAP52 increased the expression levels of GSTM1 in HRPTEpCs. Cell apoptosis assay showed that overexpression of RPSAP52 and GSTM1 decreased the apoptotic rate of HRPTEpCs under hypoxia conditions. MiR-423-5p played an opposite role and attenuated the effects of overexpressing RPSAP52 and GSTM1. Therefore, RPSAP52 may regulate miR-423-5p/GSTM1 axis to suppress hypoxia-induced HRPTEpC apoptosis.
引用
收藏
页码：1066 / 1070
页数：5
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