Nesterenkonia muleiensis sp. nov., a novel actinobacterium isolated from sap of Populus euphratica

A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2(T) was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China. RB2T was able to grow at 10-45 degrees C (optimum 35 degrees C), pH 6.0-12.0 (optimum 8.0) and with 0-12% (w/v) NaCl (optimum 1 %). The genomic DNA G+C content was 63.5% (from the genome sequence). The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9. The major fatty acids were anteiso-C-15:0 and anteiso-C-17:0 . The major polar lipids of RB2(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids. The peptidoglycan type of RB2(T) was A4 alpha, L-Lys-Gly-L-Glu. The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics. indicate that strain RB2(T) represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp. nov. is proposed. The type strain is RB2(T) (=MCCC 1K03528(T)=KCTC 49017(T)).