Development and validation of a method for simultaneous analysis of the boar taint compounds indole, skatole and androstenone in pig fat using liquid chromatography-multiple mass spectrometry

被引：38

作者：

Verheyden, K. ^{[1
]}

Noppe, H. ^{[1
]}

Aluwe, M. ^{[2
]}

Millet, S. ^{[2
]}

Bussche, J. Vanden ^{[1
]}

De Brabander, H. F. ^{[1
]}

机构：

[1] Univ Ghent, Fac Vet Med, Res Grp Vet Publ Hlth & Zoonoses, B-9820 Merelbeke, Belgium

[2] ILVO, Anim Sci Unit, B-9000 Melle, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1174卷 / 1-2期

关键词：

boar taint; LC-MSn; indole; skatole; androstenone; fat matrix;

D O I：

10.1016/j.chroma.2007.08.075

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Boar taint in entire male pigs remains a problem for fresh pork production. Since castration of pigs will be prohibited in the future on animal welfare reasons, attempts are made to detect boar taint pre and post mortem. Post mortem, techniques focus on simultaneous quantification of the three boar taint substances by one simple and reliable method. In this study a liquid chromatographic multiple mass spectrometric (LC-MSn) method has been developed and validated for the simultaneous determination of indole (2,3-benzopyrrole, ID), skatole (3-methylindole, SK) and androstenone (5 alpha-androst-16-en-3-one, AEON) in pig fat samples. Sample preparation was kept as short as possible, since a single extraction method for structurally different indols and steroids was seeked after. Analytes were extracted from the fat matrix by methanol and clean-up consisted of freezing the extract in liquid nitrogen followed by a filtration step. The analytes were chromatographically separated on a Symmetry C-18 column. Recoveries for ID, SK and AEON, as calculated from fortified fat samples using 2-methylindole (2-MID) as internal standard, were 96, 91 and 104%, respectively. However, matrix interferences were encountered determining the androstenone levels in fat. Linearity, determined in fat samples, was in the range of 50-1600 mu g kg(-1) for the indolic compounds and 125-2000 mu g kg(-1) for the steroid AEON. The correlation coefficients (R-2) of the calibration curves were 0.998 for ID, 0.997 for SK and 0.810 for AEON. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：132 / 137

页数：6

共 21 条

[1] Levels of androstenone and skatole and the occurrence of boar taint in fat from young boars [J].

Aldal, I ;

Andresen, O ;

Egeli, AK ;

Haugen, JE ;

Grodum, A ;

Fjetland, O ;

Eikaas, JLH .

LIVESTOCK PRODUCTION SCIENCE, 2005, 95 (1-2) :121-129

[2]

ALUWE M, 2007, DUTCH FOOD RES NVO, P69

[3] The problem of taint in pork .2. The influence of skatole, androstenone and indole, presented individually and in combination in a model lipid base, on odour perception [J].

AnnorFrempong, IE ;

Nute, GR ;

Whittington, FW ;

Wood, JD .

MEAT SCIENCE, 1997, 47 (1-2) :49-61

[4]

[Anonymous], P 30 INT C MEAT SCI

[5] A comparative study of boar taint in cooked and dry-cured meat [J].

Bañón, S ;

Costa, E ;

Gil, MD ;

Garrido, MD .

MEAT SCIENCE, 2003, 63 (03) :381-388

[6]

BANON S, MEAT SCI, V65

[7] An international study on the importance of androstenone and skatole for boar taint: I. Presentation of the programme and measurement of boar taint compounds with different analytical procedures [J].

Bonneau, M ;

Kempster, AJ ;

Claus, R ;

Claudi-Magnussen, C ;

Diestre, A ;

Tornberg, E ;

Walstra, P ;

Chevillon, P ;

Weiler, U ;

Cook, GL .

MEAT SCIENCE, 2000, 54 (03) :251-259

[8] Effects of raw potato starch and live weight on fat and plasma skatole, indole and androstenone levels measured by different methods in entire male pigs [J].

Chen, G. ;

Zamaratskaia, G. ;

Andersson, H. K. ;

Lundstrom, K. .

FOOD CHEMISTRY, 2007, 101 (02) :439-448

[9] Effect of hCG administration on the relationship between testicular steroids and indolic compounds in fat and plasma in entire male pigs [J].

Chen, G ;

Zamaratskaia, G ;

Madej, A ;

Lundström, K .

MEAT SCIENCE, 2006, 72 (02) :339-347

[10]

CLAUS R, 1997, ARCH LEBENSMITTELHYG, V48, P25

← 1 2 3 →