Antioxidant properties of peptide fractions from silver carp (Hypophthalmichthys molitrix) processing by-product protein hydrolysates evaluated by electron spin resonance spectrometry

被引：63
作者：
Zhong, Saiyi [1 ]
Ma, Changwei [1 ]
Lin, Young C. [2 ,3 ]
Luo, Yongkang [1 ]
机构：
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
[2] Ohio State Univ, Coll Vet Med, Lab Reprod & Mol Endocrinol, Columbus, OH 43210 USA
[3] Ohio State Univ, OSU Comprehens Canc Ctr, Columbus, OH 43210 USA
来源：
FOOD CHEMISTRY | 2011年 / 126卷 / 04期
关键词：
Silver carp by-product; Protein hydrolysates; Peptide fractions; Antioxidant activity; Electron spin resonance; ENZYME INHIBITORY PEPTIDE; FRAME PROTEIN; TEA CATECHINS; FREE-RADICALS; IN-VIVO; DIGESTS; STRESS; DAMAGE; FOOD; ESR;
D O I：
10.1016/j.foodchem.2010.12.046
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Silver carp processing by-product protein is usually discarded as an industrial solid waste. In this study the protein was recovered using a pH-shift method, after which seven commercial proteases were separately employed to prepare antioxidative hydrolysates. Among the hydrolysates, pepsin hydrolysates, which had the highest free radical-scavenging activity, were further separated into five peptide fractions, SCPH-I (> 10 kDa), SCPH-II (5-10 kDa), SCPH-III (3-5 kDa), SCPH-IV (1-3 kDa), and SCPH-V (< 1 kDa), by using ultrafiltration. The antioxidative properties of the peptide fractions were investigated, using a free radical-scavenging assay, by electron spin resonance. The results show that SCPH-V had the highest scavenging effects on DPPH (1,1-diphenyl-2-picrylhydrazyl), hydroxyl and superoxide anion radicals. SCPH-V had potent antioxidant activity in the prevention of the peroxidation of linoleic acid and alleviation of H2O2-induced oxidative stress in human intestinal epithelial caco-2 cells. The results indicated that the antioxidant capacity of silver carp by-product hydrolysates could be enhanced by ultrafiltration. (C) 2010 Elsevier Ltd. All rights reserved.
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页码：1636 / 1642
页数：7
相关论文
共 37 条
  • [1] Risk assessment of carcinogens in food
    Barlow, Susan
    Schlatter, Josef
    [J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2010, 243 (02) : 180 - 190
  • [2] BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25
  • [3] Nutritional approaches to combat oxidative stress in Alzheimer's disease
    Butterfield, DA
    Castegna, A
    Pocernich, CB
    Drake, J
    Scapagnini, G
    Calabrese, V
    [J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2002, 13 (08) : 444 - 461
  • [4] HYPEROXIA INDUCES DNA DAMAGE IN MAMMALIAN-CELLS
    CACCIUTTOLO, MA
    TRINH, L
    LUMPKIN, JA
    RAO, G
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (03) : 267 - 276
  • [5] Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein
    Chen, HM
    Muramoto, K
    Yamauchi, F
    Nokihara, K
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (09) : 2619 - 2623
  • [6] Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein
    Chen, HM
    Muramoto, K
    Yamauchi, F
    Fujimoto, K
    Nokihara, K
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (01) : 49 - 53
  • [7] Dahl MK, 1978, J DAIRY SCI, V61, P400
  • [8] THE USE OF ELECTRON-SPIN-RESONANCE TECHNIQUES TO DETECT FREE-RADICAL FORMATION AND TISSUE-DAMAGE
    DAVIES, MJ
    SLATER, TF
    [J]. PROCEEDINGS OF THE NUTRITION SOCIETY, 1988, 47 (03) : 397 - 405
  • [9] Antioxidant activity of proteins and peptides
    Elias, Ryan J.
    Kellerby, Sarah S.
    Decker, Eric A.
    [J]. CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2008, 48 (05) : 430 - 441
  • [10] *GB T, 2004, 50091242003 GBT
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