Cell biological applications of scanning near-field optical microscopy (SNOM)

被引：0

作者：

Subramaniam, V ^{[1
]}

Kirsch, AK ^{[1
]}

Jovin, TM ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Biol, D-37077 Gottingen, Germany

来源：

CELLULAR AND MOLECULAR BIOLOGY | 1998年 / 44卷 / 05期

关键词：

NSOM; FRET; energy transfer; GFP; photobleaching; cell imaging;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Scanning near-field optical microscopy (SNOM) yields high-resolution topographic and optical images and is an important technique for visualizing biological systems. We summarize the literature on SNOM of biological systems and present some of our recent applications in cellular biology. These include studies of: i) the binding of fluorescently conjugated lectins to cell surface glycoproteins on 3T3 Balb/c cells, ii) molecular interactions by fluorescence resonance energy transfer using photobleaching techniques, and iii) green fluorescent protein (GFP) expressed in bacteria.

引用

页码：689 / 700

页数：12

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