Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia

被引:51
作者
Gomes, Newton C. M. [1 ,2 ]
Flocco, Cecilia G. [3 ,4 ]
Costa, Rodrigo [5 ]
Junca, Howard [6 ,7 ]
Vilchez, Ramiro [6 ]
Pieper, Dietmar H. [6 ]
Kroegerrecklenfort, Ellen [3 ]
Paranhos, Rodolfo [8 ]
Mendonca-Hagler, Leda C. S. [9 ]
Smalla, Kornelia [3 ]
机构
[1] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
[2] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal
[3] Fed Res Ctr Cultivated Plants, Julius Kuhn Inst, Braunschweig, Germany
[4] Consejo Nacl Invest Cient & Tecnol CONICET, Buenos Aires, DF, Argentina
[5] Univ Algarve, Ctr Marine Sci CCMar, Microbial Ecol & Evolut Res Grp, Faro, Portugal
[6] Helmholtz Zentrum Infekt Forsch, Dept Microbial Pathogenesis, AG Biodegradat, Braunschweig, Germany
[7] CorpoGen Corp, Mol Genet & Biotechnol, Microbial Ecol Res Grp, Natl Res Network Genom & Bioinformat Extreme Envi, Bogota, DC, Colombia
[8] Univ Fed Rio de Janeiro, CCS, Lab Hydrobiol, Inst Biol, Rio De Janeiro, Brazil
[9] Univ Fed Rio de Janeiro, CCS, Inst Microbiol, Lab Taxon & Ecol Microorganisms, Rio De Janeiro, Brazil
关键词
mangrove; petroleum; rhizosphere; hydrocarbonoclastic bacteria; Guanabara bay; COMPLETE NUCLEOTIDE-SEQUENCE; HOST-RANGE PLASMIDS; BACTERIAL COMMUNITIES; MULTIVARIATE ANALYSES; RESISTANCE PLASMIDS; DIOXYGENASE GENES; DIESEL-OIL; BROAD-HOST; SOIL; NAPHTHALENE;
D O I
10.1111/j.1574-6941.2010.00962.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched.
引用
收藏
页码:276 / 290
页数:15
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