Olive oil mill wastewater toxicity in the marine environment: Alterations of stress indices in tissues of mussel Mytilus galloprovincialis

被引:91
作者
Danellakis, Dimitrios [1 ]
Ntaikou, Ioanna [2 ]
Kornaros, Michalis [2 ]
Dailianis, Stefanos [1 ]
机构
[1] Univ Patras, Dept Biol, Sect Anim Biol, Fac Sci, Patras 26500, Greece
[2] Univ Patras, Dept Chem Engn, Lab Engn Environm Proc, Patras 26500, Greece
关键词
Olive mill wastewater; Toxicity; Stress-indices; Mussel; Mytilus; DIGESTIVE GLAND-CELLS; LYSOSOMAL MEMBRANE STABILITY; INDUCED DNA-DAMAGE; ACETYLCHOLINESTERASE ACTIVITY; ZEBRA MUSSELS; DREISSENA-POLYMORPHA; POLLUTION BIOMARKERS; LIPID-PEROXIDATION; UNIO-TUMIDUS; TANNIC-ACID;
D O I
10.1016/j.aquatox.2010.11.015
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
This study investigated the impact of olive mill wastewater (OMW) as a pollutant of the marine environment, via the detection of stress indice alterations in mussels Mytilus galloprovincialis. Due to the absence of data concerning the levels of OMW in the receiving waters, mortality test (96 h) was first performed in order to estimate the range of OMW concentration where no mortality occurs. OMW concentrations ranging from 0.01 to 0.1% (v/v) showed no increased mortality and thus were used for the determination of pre-pathological alterations in tissues of mussels. In particular, mussels exposed to either 0.1 or 0.01% (v/v) OMW for 5 days showed significant alterations of stress indices in their tissues. Specifically, decreased neutral red retention (NRR) assay time values, inhibition of acetylcholinesterase (AChE) activity, as well as a significant increase of micronucleus (MN) frequency and DNA damage were detected in haemolymph/haemocytes and gills, compared with values measured in tissues of control mussels. The results of the present study showed that OMW disposal into the marine environment could induce pre-pathological alterations in marine organisms, before severe disturbances, such as disease, mortality, or population changes occur. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:358 / 366
页数:9
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