Detecting ligand-protein interactions inside cells using reactive peptide tags and split luciferase

被引：0

作者：

Takahashi, Tsuyoshi ^{[1
]}

Hagiwara, Hiroaki ^{[1
]}

机构：

[1] Gunma Univ, Grad Sch Sci & Technol, 1-5-1 Tenjin Cho, Kiryu, Gumma 3768515, Japan

来源：

CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 77期

关键词：

NATIVE CHEMICAL LIGATION; 19 KDA COMPONENT; STRUCTURAL BASIS; PHOSPHOPEPTIDE BINDING; ISOMERASE PIN1; INHIBITORS; RECOGNITION; REPORTER; NANOKAZ;

D O I：

10.1039/d1cc03556h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a method for detecting ligand-protein interactions occurring within cells using short peptide reactive tags appended to ligands and proteins, along with a split NanoLuc luciferase. This method can be applied to estimate the binding affinities of ligand-protein interactions and to detect the interactions of proteins with unstable synthetic ligands inside the cells.

引用

页码：9906 / 9909

页数：4

共 31 条

[1] Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells [J].

Amaike, Kazuma ;

Tamura, Tomonori ;

Hamachi, Itaru .

CHEMICAL COMMUNICATIONS, 2017, 53 (88) :11972-11983

[2] Calorimetric examination of high-affinity Src SH2 domain-tyrosyl phosphopeptide binding: Dissection of the phosphopeptide sequence specificity and coupling energetics [J].

Bradshaw, JM ;

Waksman, G .

BIOCHEMISTRY, 1999, 38 (16) :5147-5154

[3] Peptide ligands of pp60(c-src) SH2 domains: A thermodynamic and structural study [J].

Charifson, PS ;

Shewchuk, LM ;

Rocque, W ;

Hummel, CW ;

Jordan, SR ;

Mohr, C ;

Pacofsky, GJ ;

Peel, MR ;

Rodriguez, M ;

Sternbach, DD ;

Consler, TG .

BIOCHEMISTRY, 1997, 36 (21) :6283-6293

[4]

Chidley C, 2011, NAT CHEM BIOL, V7, P375, DOI [10.1038/NCHEMBIO.557, 10.1038/nchembio.557]

[5] Thermodynamics of phosphopeptide binding to the human peptidyl prolyl cis/trans isomerase Pin1 [J].

Daum, Sebastian ;

Fanghanel, Jorg ;

Wildemann, Dirk ;

Schiene-Fischer, Cordelia .

BIOCHEMISTRY, 2006, 45 (39) :12125-12135

[6] Calorimetric and structural studies of 1,2,3-trisubstituted cyclopropanes as conformationally constrained peptide inhibitors of Src SH2 domain binding [J].

Davidson, JP ;

Lubman, O ;

Rose, T ;

Waksman, G ;

Martin, SF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (02) :205-215

[7] NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells [J].

Dixon, Andrew S. ;

Schwinn, Marie K. ;

Hall, Mary P. ;

Zimmerman, Kris ;

Otto, Paul ;

Lubben, Thomas H. ;

Butler, Braeden L. ;

Binkowski, Brock F. ;

Machleidt, Thomas ;

Kirkland, Thomas A. ;

Wood, Monika G. ;

Eggers, Christopher T. ;

Encell, Lance P. ;

Wood, Keith V. .

ACS CHEMICAL BIOLOGY, 2016, 11 (02) :400-408

[8] β-Lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein-protein interactions [J].

Galarneau, A ;

Primeau, M ;

Trudeau, LE ;

Michnick, SW .

NATURE BIOTECHNOLOGY, 2002, 20 (06) :619-622

[9] Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate [J].

Hall, Mary P. ;

Unch, James ;

Binkowski, Brock F. ;

Valley, Michael P. ;

Butler, Braeden L. ;

Wood, Monika G. ;

Otto, Paul ;

Zimmerman, Kristopher ;

Vidugiris, Gediminas ;

Machleidt, Thomas ;

Robers, Matthew B. ;

Benink, Helene A. ;

Eggers, Christopher T. ;

Slater, Michael R. ;

Meisenheimer, Poncho L. ;

Klaubert, Dieter H. ;

Fan, Frank ;

Encell, Lance P. ;

Wood, Keith V. .

ACS CHEMICAL BIOLOGY, 2012, 7 (11) :1848-1857

[10] Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions [J].

Haruki, Hirohito ;

Gonzalez, Monica Rengifo ;

Johnsson, Kai .

PLOS ONE, 2012, 7 (05)

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