The Innate Immune DNA Sensing cGAS-STING Signaling Pathway Mediates Anti-PRRSV Function

被引：17

作者：

Xu, Yulin ^{[1
,2
,3
,4
]}

Zhang, Youwen ^{[1
,2
,3
,4
]}

Sun, Shaohua ^{[1
,2
,3
,4
]}

Luo, Jia ^{[1
,2
,3
,4
]}

Jiang, Sen ^{[1
,2
,3
,4
]}

Zhang, Jiajia ^{[1
,2
,3
,4
]}

Liu, Xueliang ^{[1
,2
,3
,4
]}

Shao, Qi ^{[1
,2
,3
,4
]}

Cao, Qi ^{[1
,2
,3
,4
]}

Zheng, Wanglong ^{[1
,2
,3
,4
]}

Chen, Nanhua ^{[1
,2
,3
,4
]}

Meurens, Francois ^{[5
,6
]}

Zhu, Jianzhong ^{[1
,2
,3
,4
]}

机构：

[1] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China

[2] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Jiangsu, Peoples R China

[3] Yangzhou Univ, Comparat Med Res Inst, Yangzhou 225009, Jiangsu, Peoples R China

[4] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

[5] INRAE, Oniris, BIOEPAR, F-44307 Nantes, France

[6] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Microbiol & Immunol, Saskatoon, SK S7N 5E2, Canada

来源：

VIRUSES-BASEL | 2021年 / 13卷 / 09期

基金：

中国国家自然科学基金;

关键词：

PRRSV; innate immunity; DNA sensor; cGAS-STING; antiviral activity; RIG-I; MARC-145; CELLS; VIRUS; ACTIVATION; INFECTION; REPLICATION; ANTAGONIZES; RESPONSES; DEFENSE; SYSTEM;

D O I：

10.3390/v13091829

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine reproductive and respiratory syndrome virus (PRRSV) modulates host innate immunity which plays a key role against PRRSV infection. As a RNA virus, PRRSV is mainly sensed by innate immune RNA receptors, whereas the role of innate immune DNA sensors in the PRRSV infection has not been elucidated. Here, we investigated the roles of DNA sensing cGAS-STING pathway in both PRRSV infected Marc-145 cells and porcine macrophages. The results show that in Marc-145 cells, the stable expression of STING with or without stimulations exhibited anti-PRRSV activity, and STING knockout heightened PRRSV infection. In CD163-3D4/21 porcine macrophages, either expression of STING or stimulation of cGAS-STING signaling obviously suppressed PRRSV infection, whereas in STING knockdown macrophages, the PRRSV infection was upregulated. Our results clearly demonstrate that the host cGAS-STING signal exerts an important antiviral role in PRRSV infection.

引用

页数：14

共 46 条

[1] Molecular and Cellular Mechanisms for PRRSV Pathogenesis and Host Response to Infection [J].