microRNA-589-5p modulates the expression of hemocyanin as part of the anti-WSSV immune response in Litopenaeus vannamei

被引:16
作者
Bao, Shiyuan [1 ,2 ,3 ]
Zheng, Zhihong [1 ,2 ,3 ]
Aweya, Jude Juventus [1 ,2 ,3 ]
Yao, Defu [1 ,2 ,3 ]
Li, Shengkang [1 ,2 ,3 ]
Sun, Caihui [4 ]
Hong, Yujian [4 ]
Zhang, Yueling [1 ,2 ,3 ]
机构
[1] Shantou Univ, Inst Marine Sci, Shantou 515063, Peoples R China
[2] Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, Shantou 515063, Peoples R China
[3] Shantou Univ, STU UMT Joint Shellfish Res Lab, Shantou 515063, Peoples R China
[4] Guangdong Yuequn Marine Biol Res & Dev Co Ltd, Jieyang 515200, Peoples R China
基金
中国国家自然科学基金;
关键词
Litopenaeus vannamei; Hemocyanin; miR-589-5p; WSSV; Antiviral response; SYNDROME VIRUS WSSV; INNATE IMMUNITY; REPLICATION; RECOGNITION; BIOGENESIS; INFECTION; PATHWAY; ROLES; CELLS;
D O I
10.1016/j.dci.2020.103642
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The respiratory glycoprotein, hemocyanin (HMC) has multiple immune-related functions, including antiviral activity. In this study, in silico methods were used to predict seven miRNAs targeting Litopenaeus vannamei HMC (LvHMC), out of which miR-589-5p was selected for further investigation because of its role in immune response. Transcript levels of miR-589-5p were ubiquitously distributed in all shrimp tissues examined, and significantly induced in hemocytes and hepatopancreas upon challenge with white-spot syndrome virus (WSSV) as well as by marine bacterial pathogens, which suggest that miR-589-5p is involved in shrimp immune response to pathogens. Morever, using Drosophila S2 cells stably overexpressing EGFP-LvHMC, flow cytometry and dual luciferase reporter assays, miR-589-5p was shown to significantly inhibit the in vitro expression of LvHMC. In addition, in vivo knockdown of miR-589-5p using antagomir-589-5p resulted in significant down-regulation in LvHMC expression, while overexpression of miR-589-5p using agomir-589-5p decreased the level of LvHMC expression in shrimp hemocytes and hepatopancreas. Further, the increased expression of miR-589-5p resulted in high shrimp mortality following WSSV challenge, coupled with an increase in the number of WSSV copies in hemocytes and hepatopancreas. These results suggest that miR-589-5p is involved in shrimp immune response to WSSV by negatively regulating the expression of LvHMC.
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页数:12
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