Cell surface display of salmobin, a thrombin-like enzyme from Agkistrodon halys venom on Escherichia coli using ice nucleation protein

被引：27

作者：

Jeong, HS

Yoo, SK

Kim, EJ

机构：

[1] Yonsei Univ, Coll Engn, Dept Biotechnol, Seoul 120749, South Korea

[2] Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2001年 / 28卷 / 2-3期

关键词：

cell surface display; ice nucleation protein; thrombin-like enzyme; salmobin;

D O I：

10.1016/S0141-0229(00)00315-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell surface display on Escherichia coli using ice nucleation protein was performed in order to develop a new expression system for recombinant eukaryotic proteins. Salmobin, the thrombin-like enzyme obtained from Korean snake (Agkistrodon halys) venom was displayed on the surface of Escherichia coli fused to the C-terminus of the ice nucleation protein (INP). an outer membrane protein of Pseudomonas syringae. The thrombin cleavage site was inserted between salmobin and INP. The presence of salmobin on the bacterial cell surface was verified by SDS-PAGE, Western blotting, whole cell ELISA, and immunofluorescence microscopy. After thrombin cleavage the thrombin-like enzyme activity of recombinant salmobin was tested and verified. We concluded that INP-based cell surface display can be used as a novel expression system for eukaryotic proteins. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：155 / 160

页数：6

共 27 条

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Zhao, HY
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Jiang, ZM
TOXICON, 2001, 39 (12) : 1821 - 1826
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Fan, CY
Qian, YC
Yang, SL
Gong, Y
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 1999, 47 (02): : 217 - 225
[3] Purification and Partial Characterization of a Thrombin-Like Enzyme (AH144) from Venom of Iranian Snake Agkistrodon Halys
Ghorbanpour, Mohammad
Ashtiani, Zokaee Farzin
Mirakabadi, Zare Abbas
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IRANIAN JOURNAL OF CHEMISTRY & CHEMICAL ENGINEERING-INTERNATIONAL ENGLISH EDITION, 2012, 31 (02): : 103 - 109
[4] Expression of gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, in Escherichia coli
Qing Yang
Min Li
Jianqiang Xu
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Xuyu Lei
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Biotechnology Letters, 2003, 25 : 101 - 104
[5] Expression of gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, in Escherichia coli
Yang, Q
Li, M
Xu, JQ
Bao, YM
Lei, XY
An, LJ
BIOTECHNOLOGY LETTERS, 2003, 25 (02) : 101 - 104
[6] Three-Step Column Chromatographic Method for Separation and Purification of Thrombin-Like Enzyme from Venom of Agkistrodon halys pallas
Bian Liujiao
Yang Xiaoyan
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CHINESE JOURNAL OF CHROMATOGRAPHY, 2006, 24 (02) : 135 - 139
[7] Crystal structure and activating effect on RyRs of AhV_TL-I, a glycosylated thrombin-like enzyme from Agkistrodon halys snake venom
Fuxing Zeng
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Zhongliang Zhu
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Liwen Niu
Xu Li
Maikun Teng
Archives of Toxicology, 2013, 87 : 535 - 545
[8] Crystal structure and activating effect on RyRs of AhV_TL-I, a glycosylated thrombin-like enzyme from Agkistrodon halys snake venom
Zeng, Fuxing
Shen, Bing
Zhu, Zhongliang
Zhang, Ping
Ji, Yonghua
Niu, Liwen
Li, Xu
Teng, Maikun
ARCHIVES OF TOXICOLOGY, 2013, 87 (03) : 535 - 545
[9] cDNA cloning and high-level expression of a thrombin-like enzyme from Agkistrodon acutus venom
Zha, XD
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Liu, J
Xu, KS
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, 2003, 25 (04): : 253 - 257
[10] Biochemical characterization of a thrombin-like enzyme and a fibrinolytic serine protease from snake (Agkistrodon saxatilis) venom
Koh, YS
Chung, KH
Kim, DS
TOXICON, 2001, 39 (04) : 555 - 560

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