Structures of sugar-binding peptides of Galega orientalis and G-officinalis lectins determine the choice of partner in rhizobium-legume symbiosis

被引：3

作者：

Baimiev, AK ^{[1
]}

Gubaidullin, II ^{[1
]}

Chemeris, AV ^{[1
]}

Vakhitov, VA ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Genet, Ufa Res Ctr, Ufa 450054, Russia

来源：

MOLECULAR BIOLOGY | 2005年 / 39卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

Rhizobium-leoume symbiosis; Galega; lectin genes; sequencing; carbohydrate-binding peptide;

D O I：

10.1007/s11008-005-0013-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RAPID and RFEL analyses revealed appreciable genetic heterogeneity of Rhizobium galegae bv. officinalis and R. galegae bv. orientalis, which are nitrogen-fixing symbiosis partners of Galega officinalis and G. orientalis, respectively, and do not form a single cross-inoculation group. Comparison of nucleotide and amino acid sequences for their lectins revealed relatively high general homology, testifying again to their close phylogenetic relationships. Yet the lectin region of the carbohydrate-binding peptide (CBP) proved to differ considerably, being TYCNPGWDPRDR in G. orientalis and TFYNEEWDLVIKDEH in G. officinalis. Conserved positions in the CBP were observed for amino acid residues involved in binding Ca2+ an Mn2+ and stabilizing the spatial structure of the carbohydrate-bind in a pocket. These findings confirm the role in Rhizobium-legume symbiosis for lectins and especially for their carbohydrate-binding domains.

引用

页码：90 / 97

页数：8