Agonist-induced down-regulation of α1B-adrenergic receptor in HEK293 cells transfected with α1B cDNA

被引:0
作者
Xu, KM [1 ]
Han, QD [1 ]
Tian, B [1 ]
Zhang, YY [1 ]
机构
[1] Beijing Med Univ, Inst Vasc Med, Hosp 3, Beijing 100083, Peoples R China
来源
SCIENCE IN CHINA SERIES C-LIFE SCIENCES | 1998年 / 41卷 / 03期
基金
中国国家自然科学基金;
关键词
alpha(1B)-adrenergic receptor; desensitization; down-regulation; gene expression;
D O I
10.1007/BF02895107
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
HEK293 cells stably expressing hamster ala-adrenergic receptor (alpha(1B)-AR) were used to observe the effect of norepinephrine (NE) on alpha(1B)-AR gene expression. Radioligand binding assays and RNase protection assays were used to determine alpha(1B)-AR number and the mRNA level, respectively. Exposure (2-24 h) of HEK293 cells to NE (10 mu mol) caused a decrease in alpha(1B)-AR mRNA with maximum change found at the 4th hour, and in alpha(1B)-AR density at the 24th hour. NE-induced decrease in alpha(1B)-AR mRNA was inhibited by protein kinase C (PKC) inhibitor calphostin C (0.1 mu mol) and mimicked by PKC activator PMA (1 mu mol). Nuclear run-off transcription assay showed that treatment of the cells with NE (10 mu mol) exerted no effect on the transcription rate of alpha(1B)-AR After the synthesis of new RNAs was inhibited by actinomycin D, NE could not accelerate the degradation of alpha(1B)-AR mRNA. The results suggested that in the HEK293 cells NE could induce the down-regulation of alpha(1B)-AR, and the effects were mediated by PKC pathway. NE could not alter the transcription rate of alpha(1B)-AR mRNA, but it might induce the synthesis of some factors and indirectly accelerate the degradation.
引用
收藏
页码:309 / 314
页数:6
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