An ICT-Based Mitochondria-Targeted Fluorescent Probe for Hydrogen Peroxide with a Large Turn-On Fluorescence Signal

被引：22

作者：

Liang, Xiao ^{[1
,2
]}

Zhang, Lu ^{[1
,2
]}

Xu, Xiaoyi ^{[3
]}

Qiao, Dan ^{[1
,2
]}

Shen, Tangliang ^{[1
,2
]}

Yin, Zheng ^{[1
,2
]}

Shang, Luqing ^{[1
,2
]}

机构：

[1] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

[2] Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China

[3] Tianjin Med Univ, Tianjin 300070, Peoples R China

来源：

CHEMISTRYSELECT | 2019年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

hydrogen peroxide; fluorescent probe; mitochondria-target; ICT; sensors; OXIDATIVE STRESS; H2O2; BIOLOGY; CELLS; IONS;

D O I：

10.1002/slct.201803185

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An ICT-based mitochondria-targeted fluorescent probe Mito-NP was designed and synthesized for the detection of H2O2, with radical fluorescence intensity changes (up to 120-fold) and a large Stokes shift (93 nm) in aqueous solution. There was good linear relationship (R-2=0.9983) between the concentration of H2O2 in the range of 0 to 100 mu M and the fluorescence intensity at 553 nm, with a low detection limit of 0.25 mu M (S/N=3). Mito-NP could also visualize enzymatically generated H2O2. Furthermore, the probe could detect endogenous H2O2 with good mitochondria targeting ability in living cells and could also detect the change in H2O2 levels during mitochondria-oriented apoptosis. Introduction

引用

页码：1330 / 1336

页数：7

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