Plasminogen activator inhibitor 1 expression in platelets is not influenced by the 4G/5G promoter polymorphism

被引：7

作者：

Brogren, Helen ^{[1
]}

Wallmark, Karin ^{[1
]}

Jern, Sverker ^{[1
]}

Karlsson, Lena ^{[1
]}

机构：

[1] Sahlgrens Univ Hosp, Inst Med, Clin Expt Res Lab, S-41685 Gothenburg, Sweden

来源：

THROMBOSIS RESEARCH | 2008年 / 121卷 / 06期

关键词：

fibrinolysis; plasminogen; activator inhibitor 1; platelets; mRNA; polymorphism;

D O I：

10.1016/j.thromres.2007.07.018

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In the present study we investigated the influence of the 4G/5G promoter polymorphism of the PAI-1 gene on the levels of PAI-1 mRNA and protein in platelets. After a screening of healthy mate subjects, thirty-eight subjects homozygote for either the 4G or 5G allele were investigated. mRNA levels were quantified by real-time PCR and PAI-1 antigen in platelets and plasma was analysed by ELISA. The platelet PAI-1 mRNA levels correlated significantly with the PAI-1 antigen content, but there was no association between the polymorphism and mRNA levels, or protein levels in platelets. Also, plasma levels of PAI-1 antigen were not associated with homozygosity of the 4G/5G polymorphism, but as expected BMI and triglycerides emerged as significant predictors of plasma PAI-1 levels. The importance of the 4G/5G polymorphism on PAI-1 levels is controversial and the present study shows that although levels of platelet mRNA are related to its content of PAI-1 protein, there is no association between the 4G/5G promoter polymorphism and platelet PAI-1 mRNA or protein expression. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：793 / 797

页数：5

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