Control of predators in industrial scale microalgae cultures with Pulsed Electric Fields

被引：38

作者：

Rego, D. ^{[1
]}

Redondo, L. M. ^{[2
]}

Geraldes, V. ^{[1
]}

Costa, L. ^{[1
]}

Navalho, J. ^{[1
]}

Pereira, M. T. ^{[3
]}

机构：

[1] A4F Algafuel SA, Lisbon, Portugal

[2] Lisbon Engn Super Inst, Lisbon, Portugal

[3] EPS, EnergyPulse Syst, Lisbon, Portugal

来源：

BIOELECTROCHEMISTRY | 2015年 / 103卷

关键词：

Pulsed Electric Fields; Microalgae; Industrial photobioreactor; CELL-MEMBRANES; ELECTROPORATION;

D O I：

10.1016/j.bioelechem.2014.08.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work describes the utilization of Pulsed Electric Fields to control the protozoan contamination of a microalgae culture, in an industrial 2.7 m(3) microalgae photobioreactor. The contaminated culture was treated with Pulsed Electric Fields, PEF, for 6 h with an average of 900 V/cm, 65 mu s pulses of 50 Hz. Working with recirculation, all the culture was uniformly exposed to the PEF throughout the assay. The development of the microalgae and protozoan populations was followed and the results showed that PEF is effective on the selective elimination of protozoa from microalgae cultures, inflicting on the protozoa growth halt, death or cell rupture, without affecting microalgae productivity. Specifically, the results show a reduction of the active protozoan population of 87% after 6 h treatment and 100% after few days of normal cultivation regime. At the same time, microalgae growth rate remained unaffected. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：60 / 64

页数：5

共 13 条

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