Surface Functionalization of Plasma Treated Ultrananocrystalline Diamond/Amorphous Carbon Composite Films

被引:1
作者
Koch, Hermann [1 ]
Popov, Cyril [1 ]
Kulisch, Wilhelm [2 ]
Spassov, G. [3 ]
Reithmaier, Johann Peter [1 ]
机构
[1] Univ Kassel, Inst Nanostruct Technol & Analyt, 40 Heinrich Plett Str, D-34132 Kassel, Germany
[2] Univ Kassel, Dept Math & Nat Sci, 40 Heinrich Plett Str, D-34132 Kassel, Germany
[3] Bulgarian Acad Sci, Cent Lab Photoproc, BG-1040 Sofia, Bulgaria
来源
NANOTECHNOLOGICAL BASIS FOR ADVANCED SENSORS | 2010年
关键词
Ultrananocrystalline diamond/amorphous carbon composite films; Surface modification; Surface functionalization; Surface characterization; DIAMOND; GROWTH;
D O I
10.1007/978-94-007-0903-4_48
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Diamond possesses a number of outstanding properties which make it a perspective material as platform for preparation of biosensors. The diamond surface needs to be activated before the chemical attachment of crosslinkers with which biomolecules can interact. In the current work we have investigated the modification of ultrananocrystalline diamond/amorphous carbon (UNCD/a-C) films by oxygen and ammonia plasmas. Afterwards the layers were functionalized in a further step to obtain thiol-active maleimide groups on the surface. We studied the possibility for direct binding of maleimide to terminal OH-groups on the UNCD surface and for silanization with 3-aminopropyltriethoxysilane (APTES) to obtain NH2-groups for the following attachment of sulfosuccinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (SSMCC). The thiol-bearing fluorescein-related dye 5-((2-(and-3)-S-(acetylmercapto) succinoyl) amino) fluorescein (SAMSA) was immobilized as an model biomolecule to evaluate the achieved thiol-activity by fluorescence microscopy. The results of the above mentioned surface modification and functionalization steps were investigated by Auger electron spectroscopy (AES) and contact angle measurements.
引用
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页码:463 / +
页数:3
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