Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (Fragaria vesca)

被引：7

作者：

Li, Shuangtao ^{[1
,2
,3
]}

Wang, Guixia ^{[1
,2
,3
]}

Chang, Linlin ^{[1
,2
,3
]}

Sun, Rui ^{[1
,2
,3
]}

Wu, Ruishuang ^{[1
,2
,3
]}

Zhong, Chuanfei ^{[1
,2
,3
]}

Gao, Yongshun ^{[1
,2
,3
]}

Zhang, Hongli ^{[1
,2
,3
]}

Wei, Lingzhi ^{[1
,2
,3
]}

Wei, Yongqing ^{[1
,2
,3
]}

Zhang, Yuntao ^{[1
,2
,3
]}

Dong, Jing ^{[1
,2
,3
]}

Sun, Jian ^{[1
,2
,3
]}

机构：

[1] Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

[2] Beijing Engn Res Ctr Strawberry, Beijing 100093, Peoples R China

[3] Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100093, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 19期

关键词：

tubby-like proteins; Fragaria vesca; abiotic stress; evolution pattern; expression analysis; ARABIDOPSIS; STRESS; ABA; RICE;

D O I：

10.3390/ijms231911961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight TLPs were identified in woodland strawberry (Fragaria vesca subspecies vesca 'Ruegen'). Protein structure analysis revealed that the structure of FvTLPs is highly conserved, but evolutionary and gene structure analyses revealed that the evolutionary pattern of FvTLP family members differs from that of their orthologous genes in Arabidopsis, poplar, and apple. Subcellular localization assays revealed that FvTLPs were localized to the nucleus and plasma membrane. FvTLPs showed no transcriptional activity. Yeast two-hybrid assays revealed that FvTLPs interact with specific FvSKP1s. The expression patterns of FvTLPs in different tissues and under various abiotic stresses (salt, drought, cold, and heat) and hormone treatments (ABA (abscisic acid) and MeJA (methyl jasmonate)) were determined. The expression patterns of FvTLPs indicated that they play a role in regulating growth and development and responses to abiotic stress in F. vesca. The GUS (beta-glucuronidase) activity of FvTLP1pro::GUS plants in GUS activity assays increased under salt and drought stress and abscisic acid treatment. The results of this study provide new insights into the molecular mechanisms underlying the functions of TLPs.

引用

页数：16

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