An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate

被引:36
作者
Teotia, S [1 ]
Khare, SK [1 ]
Gupta, MN [1 ]
机构
[1] Indian Inst Technol, Dept Chem, New Delhi 110016, India
关键词
sweet potato; beta-amylase; affinity precipitation; alginate; enzyme purification;
D O I
10.1016/S0141-0229(01)00338-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
beta -amylases are used in production of maltose syrup. It is shown that sweet potato beta -amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of beta -amylases. (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:792 / 795
页数:4
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