Thymosin beta 4 is overexpressed in human pancreatic cancer cells and stimulates proinflammatory cytokine secretion and JNK activation

被引：51

作者：

Zhang, Yuqing ^{[1
]}

Feurino, Louis W. ^{[1
]}

Zhai, Qihui ^{[2
]}

Wang, Hao ^{[1
]}

Fisher, William E. ^{[1
]}

Chen, Changyi ^{[1
]}

Yao, Qizhi ^{[1
]}

Li, Min ^{[1
]}

机构：

[1] Baylor Coll Med, Michael E DeBakey Dept Surg, Mol Surgeon Res Ctr, Elkins Pancreas Ctr, Houston, TX 77030 USA

[2] Methodist Hosp, Dept Pathol, Houston, TX 77030 USA

来源：

CANCER BIOLOGY & THERAPY | 2008年 / 7卷 / 03期

关键词：

thymosin beta 4; overexpression; tumor tissue; cytokine; proinflammation; JNK activation; pancreatic cancer;

D O I：

10.4161/cbt.7.3.5415

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Thymosin beta 4 ( T beta 4) has been shown to be associated with tumor metastasis and angiogenesis; however, its role in pancreatic cancer has not been understood. In the current study, we examined the expression of T beta 4 in pancreatic cancer cells, and determined the effect of exogenous T beta 4 on cytokine secretion, and signal transduction in human pancreatic cancer cells. Results: Pancreatic cancer cell lines expressed higher amount of T beta 4 mRNA than normal human pancreatic ductal epithelium ( HPDE) cells. Exogenous T beta 4 increased the secretion of proinflammatory cytokines IL-6, IL-8 and MCP-1 in Panc-1 cells. In addition, T beta 4 activated Jun N-terminal Kinase ( JNK) signaling pathways in pancreatic cancer cells. Methods: The mRNA levels of T beta 4 were determined by realtime RT PCR. Phosphorylation of JNK in pancreatic cancer cells was determined using Bio-Plex phosphoprotein assay. The expression of cytokines in human pancreatic cancer cell lines was determined with Bio-Plex cytokine assay. Conclusions: T beta 4 might be involved in stimulating human pancreatic cancer progression by promoting proinflammatory cytokine environment and activating JNK signaling pathway. Targeting T beta 4 and related molecules may be a novel therapeutic strategy for pancreatic cancer.

引用

页码：419 / 423

页数：5

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