Comparison of liquid-liquid extraction, microextraction and ultrafiltration for measuring free concentrations of testosterone and phenytoin

被引：3
作者：
Cibotaru, Dorina [1 ]
Celestin, Marie N. [2 ]
Kane, Michael P. [2 ]
Musteata, Florin M. [1 ]
机构：
[1] Albany Coll Pharm & Hlth Sci, Dept Pharmaceut Sci, 106 New Scotland Ave, Albany, NY 12208 USA
[2] Albany Coll Pharm & Hlth Sci, Dept Pharm Practice, 106 New Scotland Ave, Albany, NY 12208 USA
来源：
BIOANALYSIS | 2021年 / 14卷 / 04期
基金：
美国国家卫生研究院;
关键词：
albumin; free concentration; liquid-liquid extraction; phenytoin; plasma protein binding; solid-phase microextraction; testosterone; ultrafiltration; PLASMA-PROTEIN BINDING; VANCOMYCIN;
D O I：
10.4155/bio-2021-0249
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: The purpose of the study was to find methods suitable for measuring the free concentrations of testosterone and phenytoin. Materials & methods: Sample solutions of the compounds in buffer and human albumin were processed using liquid-liquid extraction, microextraction and ultrafiltration and analyzed by LC-MS/MS. Results: Liquid-liquid extraction with dibutyl phthalate provided complete extraction from buffer solutions and partial extraction from albumin samples. Spintip C18 devices provided exhaustive extraction from buffer and albumin samples. Spintip C8 devices offered complete extraction from buffer and approximately 50% recovery from albumin samples. Centrifree ultrafiltration devices showed high recovery of free concentrations from all the samples, while Amicon and Nanosep devices provided partial recovery. Conclusion: Spintip C8 and Centrifree devices proved useful for measuring free concentrations.
引用
收藏
页码：195 / 204
页数：10
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