New insights into ToF-SIMS imaging in osteoporotic bone research

被引:11
作者
Kern, Christine [1 ]
Ray, Seemun [2 ]
Gelinsky, Michael [3 ,4 ]
Bellew, Allen T. [5 ]
Pirkl, Alexander [6 ]
Rohnke, Marcus [1 ]
机构
[1] Justus Liebig Univ Giessen, Inst Phys Chem, Heinrich Buff Ring 17, D-35392 Giessen, Germany
[2] Justus Liebig Univ, Expt Trauma Surg, Aulweg 128, D-35392 Giessen, Germany
[3] Tech Univ Dresden, Ctr Translat Bone Joint & Soft Tissue Res, Med Fac, Fetscherstr 74, D-01307 Dresden, Germany
[4] Tech Univ Dresden, Univ Hosp, Fetscherstr 74, D-01307 Dresden, Germany
[5] Ionoptika Ltd, Unit B6 Millbrook Close, Chandlers Ford, England
[6] IONTOF GmbH, Heisenbergstr 15, D-48149 Munster, Germany
关键词
CALCIUM-PHOSPHATE CEMENTS; ION MASS-SPECTROMETRY; PRINCIPAL COMPONENT ANALYSIS; STRONTIUM RANELATE; ADSORBED PROTEINS; CORTICAL BONE; IN-VITRO; IMPLANT; BIOLOGY; MATRIX;
D O I
10.1116/6.0000051
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The present work focuses on the application of time-of-flight secondary ion mass spectrometry (ToF-SIMS) in osteoporotic bone research. In order to demonstrate the benefit, the authors present concrete application examples of ToF-SIMS in three different areas of bone research. ToF-SIMS as a mass spectrometric imaging technique allows simultaneous visualization of mineralized and nonmineralized bone tissue as well as implanted biomaterials and bone implant interphases. In the first example, the authors show that it is possible to study the incorporation and distribution of different components released from bone filler materials into bone with a single mass spectrometric measurement. This not only enables imaging of nonstained bone cross sections but also provides further insights beyond histologically obtained information. Furthermore, they successfully identified several mass fragments as markers for newly formed cartilage tissue and growth joint in bone. Different modes of ToF-SIMS as well as different SIMS instruments (IONTOF's TOF.SIMS 5 and M6 Hybrid SIMS, Ionoptika's J105) were used to identify these mass signals and highlight the high versatility of this method. In the third part, bone structure of cortical rat bone was investigated from bone sections embedded in technovit (polymethyl methacrylate, PMMA) and compared to cryosections. In cortical bone, they were able to image different morphological features, e.g., concentric arrangement of collagen fibers in so-called osteons as well as Haversian canals and osteocytes. In summary, the study provides examples of application and shows the strength of ToF-SIMS as a promising analytical method in the field of osteoporotic bone research.
引用
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页数:22
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共 99 条
[1]   FUNCTION OF OSTEOCYTES IN BONE [J].
AARDEN, EM ;
BURGER, EH ;
NIJWEIDE, PJ .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 55 (03) :287-299
[2]   A new metaphyseal bone defect model in osteoporotic rats to study biomaterials for the enhancement of bone healing in osteoporotic fractures [J].
Alt, Volker ;
Thormann, Ulrich ;
Ray, Seemun ;
Zahner, Daniel ;
Duerselen, Lutz ;
Lips, Katrin ;
El Khassawna, Thaqif ;
Heiss, Christian ;
Riedrich, Alina ;
Schlewitz, Gudrun ;
Ignatius, Anita ;
Kampschulte, Marian ;
von Dewitz, Helena ;
Heinemann, Sascha ;
Schnettler, Reinhard ;
Langheinrich, Alexander .
ACTA BIOMATERIALIA, 2013, 9 (06) :7035-7042
[3]   Accelerated bone ingrowth by local delivery of strontium from surface functionalized titanium implants [J].
Andersen, Ole Z. ;
Offermanns, Vincent ;
Sillassen, Michael ;
Almtoft, Klaus P. ;
Andersen, Inge H. ;
Sorensen, Soren ;
Jeppesen, Christian S. ;
Kraft, David C. E. ;
Bottiger, Jorgen ;
Rasse, Michael ;
Kloss, Frank ;
Foss, Morten .
BIOMATERIALS, 2013, 34 (24) :5883-5890
[4]   Identification of a lipid-related peak set to enhance the interpretation of TOF-SIMS data from model and cellular membranes [J].
Anderton, Christopher R. ;
Vaezian, Bita ;
Lou, Kaiyan ;
Frisz, Jessica F. ;
Kraft, Mary L. .
SURFACE AND INTERFACE ANALYSIS, 2012, 44 (03) :322-333
[5]   High energy gas cluster ions for organic and biological analysis by time-of-flight secondary ion mass spectrometry [J].
Angerer, Tina B. ;
Blenkinsopp, Paul ;
Fletcher, John S. .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2015, 377 :591-598
[6]   XPS and ToF-SIMS Investigation of α-Helical and β-Strand Peptide Adsorption onto SAMs [J].
Apte, Julia S. ;
Collier, Galen ;
Latour, Robert A. ;
Gamble, Lara J. ;
Castner, David G. .
LANGMUIR, 2010, 26 (05) :3423-3432
[7]   Strontium distribution in bones and tissues of strontium ranelate-administrated rats [J].
Aranyosiova, M. ;
Stancikova, M. ;
Rovensky, J. ;
Velic, D. .
SURFACE AND INTERFACE ANALYSIS, 2011, 43 (1-2) :306-309
[8]   Osteocyte lacunar properties in rat cortical bone: Differences between lamellar and central bone [J].
Bach-Gansmo, Fiona Linnea ;
Weaver, James C. ;
Jensen, Mads Hartmann ;
Leemreize, Hanna ;
Mader, Kevin Scott ;
Stampanoni, Marco ;
Brueel, Annemarie ;
Thomsen, Jesper Skovhus ;
Birkedal, Henrik .
JOURNAL OF STRUCTURAL BIOLOGY, 2015, 191 (01) :59-67
[9]   Calcified Cartilage Islands in Rat Cortical Bone [J].
Bach-Gansmo, Fiona Linnea ;
Irvine, Sarah Catherine ;
Bruel, Annemarie ;
Thomsen, Jesper Skovhus ;
Birkedal, Henrik .
CALCIFIED TISSUE INTERNATIONAL, 2013, 92 (04) :330-338
[10]   Biglycan modulates angiogenesis and bone formation during fracture healing [J].
Berendsen, Agnes D. ;
Pinnow, Emily L. ;
Maeda, Azusa ;
Brown, Aaron C. ;
McCartney-Francis, Nancy ;
Kram, Vardit ;
Owens, Rick T. ;
Robey, Pamela G. ;
Holmbeck, Kenn ;
de Castro, Luis F. ;
Kilts, Tina M. ;
Young, Marian F. .
MATRIX BIOLOGY, 2014, 35 :223-231