The Diversified O-Superfamily in Californiconus californicus Presents a Conotoxin with Antimycobacterial Activity

被引：14

作者：

Bernaldez-Sarabia, Johanna ^{[1
]}

Figueroa-Montiel, Andrea ^{[1
]}

Duenas, Salvador ^{[1
]}

Cervantes-Luevano, Karla ^{[1
]}

Beltran, Jesus A. ^{[2
]}

Ortiz, Ernesto ^{[3
]}

Jimenez, Samanta ^{[1
]}

Possani, Lourival D. ^{[3
]}

Paniagua-Solis, Jorge F. ^{[4
]}

Gonzalez-Canudas, Jorge ^{[5
]}

Licea-Navarro, Alexei ^{[1
]}

机构：

[1] CICESE, Dept Innovac Biomed, Carretera Ensenada Tijuana 3918, Ensenada 22860, Baja California, Mexico

[2] CICESE, Dept Ciencias Computac, Carretera Ensenada Tijuana 3918, Ensenada 22860, Baja California, Mexico

[3] UNAM, IBT, Dept Med Mol & Bioproc, Cuernavaca 62210, Morelos, Mexico

[4] Teraclon IDF SL, Parque Tecnol Madrid, Madrid 28760, Spain

[5] Lab Silanes SA CV, Mexico City 11000, DF, Mexico

来源：

TOXINS | 2019年 / 11卷 / 02期

关键词：

Conus californicus; Californiconus californicus; O-superfamily conotoxin; transcriptome; tuberculosis; anti-TB; antimycobacterial; multidrug-resistant tuberculosis; CONE SNAIL;

D O I：

10.3390/toxins11020128

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Californiconus californicus, previously named Conus californicus, has always been considered a unique species within cone snails, because of its molecular, toxicological and morphological singularities; including the wide range of its diet, since it is capable of preying indifferently on fish, snails, octopus, shrimps, and worms. We report here a new cysteine pattern conotoxin assigned to the O1-superfamily capable of inhibiting the growth of Mycobacterium tuberculosis (Mtb). The conotoxin was tested on a pathogen reference strain (H37Rv) and multidrug-resistant strains, having an inhibition effect on growth with a minimal inhibitory concentration (MIC) range of 3.52-0.22 M, similar concentrations to drugs used in clinics. The peptide was purified from the venom using reverse phase high-performance liquid chromatography (RP-HPLC), a partial sequence was constructed by Edman degradation, completed by RACE and confirmed with venom gland transcriptome. The 32-mer peptide containing eight cysteine residues was named O1_cal29b, according to the current nomenclature for this type of molecule. Moreover, transcriptomic analysis of O-superfamily toxins present in the venom gland of the snail allowed us to assign several signal peptides to O2 and O3 superfamilies not described before in C. californicus, with new conotoxins frameworks.

引用

页数：13

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