Cation exchange resin pretreatment enhancing methane production from anaerobic digestion of waste activated sludge

The application of anaerobic digestion (AD) to treat waste activated sludge (WAS) still exhibits some limitations, such as low methane production. In this study, cation exchange resin (CER) pretreatment was explored to enhance the efficiency of the AD of WAS. Based on the response surface methodology, the optimal conditions for CER pretreatment were reaction time of 7.4 h, 33.8 g CER (wet weight) /g volatile solids and sludge total solids of 2.4%. Under these optimal CER pretreatment conditions, approximately 30% of metals were removed from the WAS, particularly organic-binding metals. This metal removal disrupted the structures of extracellular polymer substances and led to sludge deflocculation, thereby releasing large amounts of organic substances from the sludge solids. Batch AD experiments showed that CER pretreatment increased the maximal production of volatile fatty acids and methane by 565.7% and 80.5%, respectively. Additionally, CER pretreatment promoted each stage of AD (i.e. solubilisation, hydrolysis, acidification and methanation) and the corresponding activities of key enzymes. Experimental results for semi-continuous AD further confirmed that CER pretreatment enhanced the proportion of methane in the biogas (from 62.75 +/- 2.14% to 73.96 +/- 0.99%) and the production of methane. An analysis of changes in the microbial communities demonstrated that CER pretreatment enhanced the abundance of microorganisms involved in hydrolysis, acidification and acetification and changed the major methanogenic pathway from acetoclastic methanogens to methylotrophic methanogens. These findings are expected to provide a reference for developing new pretreatment methods for enhancing anaerobic biodegradability of organic matters.