A comparison of sequence resolution on plant chromosomes: PRINS versus FISH

被引：22

作者：

Menke, M ^{[1
]}

Fuchs, J ^{[1
]}

Schubert, I ^{[1
]}

机构：

[1] IPK, Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 1998年 / 97卷 / 08期

关键词：

plant chromosomes; primed in situ; DNA labelling; fluorescent in situ hybridization; repetitive sequences;

D O I：

10.1007/s001220051024

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The resolution of the chromosomal positions of six high- and one low-copy sequences by oligonucleotide-primed in situ (PRINS) labelling was compared with corresponding data obtained after fluorescent in situ hybridization (FISH) on field-bean and barley chromosomes. While PRINS proved to be suitable for the rapid detection of high-copy tandem repeats: at the same loci as those revealed by FISH, no clear PRINS signal was obtained for the low-copy family of vicilin genes at their locus on field-bean chromosome II. This indicates that localization of short target sequences by primer extension via Taq polymerase in situ does not yet provide a resolution equal, or superior, to FISH on plant chromosomes. Therefore, the use of a cocktail of chromosome-specific single-copy sequences as primers for PRINS is no alternative for the not as yet feasible chromosome painting in plants.

引用

页码：1314 / 1320

页数：7

共 44 条

[1] PRIMER-INDUCED IN-SITU HYBRIDIZATION TO PLANT CHROMOSOMES [J].

ABBO, S ;

MILLER, TE ;

KING, IP .

GENOME, 1993, 36 (05) :815-817

[2] PRIMER-MEDIATED IN-SITU DETECTION OF THE B-HORDEIN GENE-CLUSTER ON BARLEY CHROMOSOME 1H [J].

ABBO, S ;

DUNFORD, RP ;

MILLER, TE ;

READER, SM ;

KING, IP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :11821-11824

[3] CHARACTERIZATION OF RELIC DNA FROM BARLEY GENOME [J].

BELOSTOTSKY, DA ;

ANANIEV, EV .

THEORETICAL AND APPLIED GENETICS, 1990, 80 (03) :374-380

[4] LOCALIZATION OF SINGLE-COPY GENE BY PRINS TECHNIQUE [J].

CINTI, C ;

SANTI, S ;

MARALDI, NM .

NUCLEIC ACIDS RESEARCH, 1993, 21 (24) :5799-5800

[5] DISTRIBUTION OF HETEROCHROMATIN IN A RECONSTRUCTED KARYOTYPE OF VICIA-FABA AS IDENTIFIED BY BANDING- AND DNA-LATE REPLICATION PATTERNS [J].

DOBEL, P ;

SCHUBERT, I ;

RIEGER, R .

CHROMOSOMA, 1978, 69 (02) :193-209

[6] LOCALIZATION OF VICILIN GENES ON FIELD BEAN CHROMOSOME-II BY FLUORESCENT IN-SITU HYBRIDIZATION [J].

FUCHS, J ;

JOOS, S ;

LICHTER, P ;

SCHUBERT, I .

JOURNAL OF HEREDITY, 1994, 85 (06) :487-488

[7] LOCALIZATION OF SEED PROTEIN GENES ON METAPHASE CHROMOSOMES OF VICIA-FABA VIA FLUORESCENCE IN-SITU HYBRIDIZATION [J].

FUCHS, J ;

SCHUBERT, I .

CHROMOSOME RESEARCH, 1995, 3 (02) :94-100

[8] Chromosome 'painting' in plants - A feasible technique? [J].

Fuchs, J ;

Houben, A ;

Brandes, A ;

Schubert, I .

CHROMOSOMA, 1996, 104 (05) :315-320

[9] Differentiation of field bean heterochromatin by in situ hybridization with a repeated Fok I sequence [J].

Fuchs, J. ;

Pich, U. ;

Meister, A. ;

Schubert, I. .

CHROMOSOME RESEARCH, 1994, 2 (01) :25-28

[10] Molecular-cytogenetic characterization of the Vicia faba genome - heterochromatin differentiation, replication patterns and sequence localization [J].

Fuchs, J ;

Strehl, S ;

Brandes, A ;

Schweizer, D ;

Schubert, I .

CHROMOSOME RESEARCH, 1998, 6 (03) :219-230

← 1 2 3 4 5 →