Dynamic visualization of local protein synthesis in hippocampal neurons

被引:468
作者
Aakalu, G [1 ]
Smith, WB [1 ]
Nguyen, N [1 ]
Jiang, CG [1 ]
Schuman, EM [1 ]
机构
[1] CALTECH, Howard Hughes Med Inst, Div Biol 216 76, Pasadena, CA 91125 USA
关键词
D O I
10.1016/S0896-6273(01)00295-1
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Using pharmacological approaches, several recent studies suggest that local protein synthesis is required for synaptic plasticity. Convincing demonstrations of bona fide dendritic protein synthesis in mammalian neurons are rare, however. We developed a protein synthesis reporter in which the coding sequence of green fluorescent protein is flanked by the 5' and 3' untranslated regions from CAMKII-alpha, conferring both dendritic mRNA localization and translational regulation. In cultured hippocampal neurons, we show that BDNF, a growth factor involved in synaptic plasticity, stimulates protein synthesis of the reporter in intact, mechanically, or "optically" isolated dendrites. The stimulation of protein synthesis is blocked by anisomycin and not observed in untreated neurons. In addition, dendrites appear to possess translational hot spots, regions near synapses where protein synthesis consistently occurs over time.
引用
收藏
页码:489 / 502
页数:14
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