Determination of scutellarin in Erigeron breviscapus extract by liquid chromatography-tandem mass spectrometry

被引：47

作者：

Qu, J ^{[1
]}

Wang, YM ^{[1
]}

Luo, GA ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Sch Life Sci & Technol, Beijing 100084, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 919卷 / 02期

关键词：

Erigeron breviscapus; scutellarin;

D O I：

10.1016/S0021-9673(01)00849-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative assay for scutellarin by LC-MS-MS (negative ion mode) was developed. The scutellarin was extracted from dry Erigeron breviscapus. Significant ion suppression was observed, which could be eliminated by increasing the turboionspray interface temperature to 350 degreesC and by 1000-fold dilution of the extract with solvent. The calibration curve of scutellarin showed excellent linearity over a, wide concentration range (0.01-100 mug/ml) (r=0.998), and the limit of detection was 15 pg/ml using a 10-mul injection volume. The analysis time was 4 min/sample. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：437 / 441

页数：5

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