Esophageal Microbiome in Eosinophilic Esophagitis

被引：119

作者：

Harris, J. Kirk ^{[1
]}

Fang, Rui ^{[2
]}

Wagner, Brandie D. ^{[1
,2
]}

Choe, Ha Na ^{[3
]}

Kelly, Caleb J. ^{[3
]}

Schroeder, Shauna ^{[3
]}

Moore, Wendy ^{[3
]}

Stevens, Mark J. ^{[1
]}

Yeckes, Alyson ^{[3
]}

Amsden, Katie ^{[4
,5
,6
]}

Kagalwalla, Amir F. ^{[4
,5
,6
]}

Zalewski, Angelika ^{[7
]}

Hirano, Ikuo ^{[7
]}

Gonsalves, Nirmala ^{[7
]}

Henry, Lauren N. ^{[3
]}

Masterson, Joanne C. ^{[3
]}

Robertson, Charles E. ^{[8
]}

Leung, Donald Y. ^{[9
]}

Pace, Norman R. ^{[8
]}

Ackerman, Steven J. ^{[10
]}

Furuta, Glenn T. ^{[3
]}

Fillon, Sophie A. ^{[3
]}

机构：

[1] Univ Colorado, Sch Med, Div Pulmonol, Aurora, CO USA

[2] Univ Colorado, Colorado Sch Publ Hlth, Dept Biostat & Informat, Aurora, CO USA

[3] Univ Colorado, Childrens Hosp Colorado, Sch Med,Sect Pediat Gastroenterol, Digest Hlth Inst,Mucosal Inflammat Program,Gastro, Aurora, CO 80045 USA

[4] Ann & Robert H Lurie Childrens Hosp Chicago, Dept Pediat, Div Gastroenterol Hepatol & Nutr, Chicago, IL USA

[5] Northwestern Univ, Chicago, IL 60611 USA

[6] John H Stroger Hosp Cook Cty, Dept Pediat, Chicago, IL USA

[7] Northwestern Univ, Feinberg Sch Med, Dept Med, Div Gastroenterol & Hepatol, Chicago, IL 60611 USA

[8] Univ Colorado, Mol Cellular & Dev Biol, Boulder, CO 80309 USA

[9] Natl Jewish Hlth, Dept Pediat, Denver, CO USA

[10] Univ Illinois, Dept Biochem & Mol Genet, Chicago, IL USA

来源：

PLOS ONE | 2015年 / 10卷 / 05期

基金：

美国国家卫生研究院;

关键词：

GUT MICROBIOME; CONSENSUS RECOMMENDATIONS; CHILDREN; ADULTS; DIET;

D O I：

10.1371/journal.pone.0128346

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objective The microbiome has been implicated in the pathogenesis of a number of allergic and inflammatory diseases. The mucosa affected by eosinophilic esophagitis (EoE) is composed of a stratified squamous epithelia and contains intraepithelial eosinophils. To date, no studies have identified the esophageal microbiome in patients with EoE or the impact of treatment on these organisms. The aim of this study was to identify the esophageal microbiome in EoE and determine whether treatments change this profile. We hypothesized that clinically relevant alterations in bacterial populations are present in different forms of esophagitis. Design In this prospective study, secretions from the esophageal mucosa were collected from children and adults with EoE, Gastroesophageal Reflux Disease (GERD) and normal mucosa using the Esophageal String Test (EST). Bacterial load was determined using quantitative PCR. Bacterial communities, determined by 16S rRNA gene amplification and 454 pyrosequencing, were compared between health and disease. Results Samples from a total of 70 children and adult subjects were examined. Bacterial load was increased in both EoE and GERD relative to normal subjects. In subjects with EoE, load was increased regardless of treatment status or degree of mucosal eosinophilia compared with normal. Haemophilus was significantly increased in untreated EoE subjects as compared with normal subjects. Streptococcus was decreased in GERD subjects on proton pump inhibition as compared with normal subjects. Conclusions Diseases associated with mucosal eosinophilia are characterized by a different microbiome from that found in the normal mucosa. Microbiota may contribute to esophageal inflammation in EoE and GERD.

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