Arctiin Prevents LPS-Induced Acute Lung Injury via Inhibition of PI3K/AKT Signaling Pathway in Mice

被引:59
作者
Zhou, Bo [1 ]
Weng, Guohu [2 ]
Huang, Zhengxin [3 ]
Liu, Tao [2 ]
Dai, Feiyue [1 ]
机构
[1] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Intens Care Unit, Changsha, Hunan, Peoples R China
[2] Hainan Prov Hosp TCM, Dept Neurol, Haikou, Hainan, Peoples R China
[3] Hainan Prov Hosp TCM, Dept Cardiol, Haikou, Hainan, Peoples R China
关键词
LPS; lung injury; NF-B; PI3K; arctiin; NF-KAPPA-B; INFLAMMATORY CYTOKINES; PERSISTENT ELEVATION; ACTIVATION; PREDICTS; ARDS; AKT;
D O I
10.1007/s10753-018-0856-x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Arctiin is a lignin isolated from Arctium lappa which has been known to have anti-viral and anti-inflammatory effects. The aim of this study is to explore the protective effect of arctiin on lipopolysaccharide (LPS)-induced inflammatory responses in acute lung injury (ALI) model of mice. Male BALB/c mice were pretreated with commercial arctiin (10, 20, and 40mg/kg) 1h prior to LPS challenge. Twelve hours later, airway inflammation was assessed. We assessed the effects of arctiin on the LPS-induced production of TNF-, IL-6, and IL-1 in the bronchoalveolar lavage fluid (BALF). The lung wet-to-dry weight ratio, myeloperoxidase (MPO) activity, and inflammatory signaling pathway were also detected. Our results showed that arctiin not only significantly ameliorated LPS-stimulated lung histopathological changes but also reduced the lung MPO activity. Arctiin also dramatically decreased the production of TNF-, IL-1, and IL-6 in the BALF. In addition, arctiin significantly inhibited LPS-induced PI3K/Akt phosphorylation as well as NF-B activation. In conclusion, our results suggested that arctiin protected against LPS-induced ALI through inhibiting PI3K/AKT/NF-B signaling pathway.
引用
收藏
页码:2129 / 2135
页数:7
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