Synergistic effect of HIF-1 and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

被引:58
作者
Chen, Ya-Fang [1 ,2 ]
Pandey, Sudhir [1 ]
Day, Cecilia Hsuan [3 ]
Chen, Yu-Feng [4 ]
Jiang, Ai-Zhi [1 ]
Ho, Tsung-Jung [5 ]
Chen, Ray-Jade [6 ]
Padma, Vijaya V. [7 ]
Kuo, Wei-Wen [8 ]
Huang, Chih-Yang [1 ,5 ,9 ,10 ]
机构
[1] China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan
[2] Taichung Vet Gen Hosp, Dept Obstet & Gynecol, Taichung, Taiwan
[3] MeiHo Univ, Dept Nursing, Pingtung, Taiwan
[4] Yuan Rung Hosp, Cardiol Sect, Yuanlin, Taiwan
[5] China Med Univ, Sch Med, Coll Med, Taichung, Taiwan
[6] Taipei Med Univ, Sch Med, Dept Surg, Coll Med, Taipei, Taiwan
[7] Bharathiar Univ, Dept Biotechnol, Coimbatore, Tamil Nadu, India
[8] China Med Univ, Dept Biol Sci & Technol, Taichung, Taiwan
[9] Ton Duc Thang Univ, Fac Sci Appl, Tan Phong Ward, Ho Chi Minh City, Vietnam
[10] Asia Univ, Dept Biol Sci & Technol, Taichung, Taiwan
关键词
BCL2; Adenovirus E1B Nineteen kilo Dalton Interacting Protein 3 (BNIP3); diabetic hyperglycemic; forkhead box O3 (FoxO3); hyperglycemia; hypoxia-inducible factor-1 alpha (HIF-1); ischemia hypoxia; CARDIAC MYOCYTE DEATH; HYPOXIC ACCUMULATION; BNIP3; PROTEIN; PATHWAYS; STRESS; HIF-1-ALPHA; INHIBITION; INFARCTION; INDUCTION;
D O I
10.1002/jcp.26235
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1 and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1 and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1 and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.
引用
收藏
页码:3660 / 3671
页数:12
相关论文
共 41 条
  • [1] FOXO3a is activated in response to hypoxic stress and inhibits HiF1-induced apoptosis via regulation of CITED2
    Bakker, Walbert J.
    Harris, Isaac S.
    Mak, Tak W.
    [J]. MOLECULAR CELL, 2007, 28 (06) : 941 - 953
  • [2] ROLE OF OXIDATIVE STRESS IN DEVELOPMENT OF COMPLICATIONS IN DIABETES
    BAYNES, JW
    [J]. DIABETES, 1991, 40 (04) : 405 - 412
  • [3] Glucose-Induced O2 Consumption Activates Hypoxia Inducible Factors 1 and 2 in Rat Insulin-Secreting Pancreatic Beta-Cells
    Bensellam, Mohammed
    Duvillie, Bertrand
    Rybachuk, Galyna
    Laybutt, D. Ross
    Magnan, Christophe
    Guiot, Yves
    Pouyssegur, Jacques
    Jonas, Jean-Christophe
    [J]. PLOS ONE, 2012, 7 (01):
  • [4] Expression of the gene encoding the proapoptotic Nip3 protein is induced by hypoxia
    Bruick, RK
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (16) : 9082 - 9087
  • [5] Programmed myocyte cell death affects the viable myocardium after infarction in rats
    Cheng, W
    Kajstura, J
    Nitahara, JA
    Li, BS
    Reiss, K
    Liu, Y
    Clark, WA
    Krajewski, S
    Reed, JC
    Olivetti, G
    Anversa, P
    [J]. EXPERIMENTAL CELL RESEARCH, 1996, 226 (02) : 316 - 327
  • [6] BNIP3 subfamily BH3-only proteins: mitochondrial stress sensors in normal and pathological functions
    Chinnadurai, G.
    Vijayalingam, S.
    Gibson, S. B.
    [J]. ONCOGENE, 2008, 27 (Suppl 1) : S114 - S127
  • [7] Inhibition of hypoxia inducible factor 1α causes oxygen-independent cytotoxicity and induces p53 independent apoptosis in glioblastoma cells
    Dai, S
    Huang, ML
    Hsu, CY
    Chao, KSC
    [J]. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2003, 55 (04): : 1027 - 1036
  • [8] Hypoxia-induced increase in the production of extracellular matrix proteins in systemic sclerosis
    Distler, Joerg H. W.
    Juengel, Astrid
    Pileckyte, Margarita
    Zwerina, Jochen
    Michel, Beat A.
    Gay, Renate E.
    Kowal-Bielecka, Otylia
    Matucci-Cerinic, Marco
    Schett, Georg
    Marti, Hugo H.
    Gay, Steffen
    Distler, Oliver
    [J]. ARTHRITIS AND RHEUMATISM, 2007, 56 (12): : 4203 - 4215
  • [9] Inhibition of ischemic cardiomyocyte apoptosis through targeted ablation of Bnip3 restrains postinfarction remodeling in mice
    Diwan, Abhinav
    Krenz, Maike
    Syed, Faisal M.
    Wansapura, Janaka
    Ren, Xiaoping
    Koesters, Andrew G.
    Li, Hairong
    Kirshenbaum, Lorrie A.
    Hahn, Harvey S.
    Robbins, Jeffrey
    Jones, W. Keith
    Dorn, Gerald W., II
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2007, 117 (10) : 2825 - 2833
  • [10] Cardiac reanimation: targeting cardiomyocyte death by BNIP3 and NIX/BNIP3L
    Dorn, G. W., II
    Kirshenbaum, L. A.
    [J]. ONCOGENE, 2008, 27 (Suppl 1) : S158 - S167