Synergistic effect of HIF-1 and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

被引：58

作者：

Chen, Ya-Fang ^{[1
,2
]}

Pandey, Sudhir ^{[1
]}

Day, Cecilia Hsuan ^{[3
]}

Chen, Yu-Feng ^{[4
]}

Jiang, Ai-Zhi ^{[1
]}

Ho, Tsung-Jung ^{[5
]}

Chen, Ray-Jade ^{[6
]}

Padma, Vijaya V. ^{[7
]}

Kuo, Wei-Wen ^{[8
]}

Huang, Chih-Yang ^{[1
,5
,9
,10
]}

机构：

[1] China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan

[2] Taichung Vet Gen Hosp, Dept Obstet & Gynecol, Taichung, Taiwan

[3] MeiHo Univ, Dept Nursing, Pingtung, Taiwan

[4] Yuan Rung Hosp, Cardiol Sect, Yuanlin, Taiwan

[5] China Med Univ, Sch Med, Coll Med, Taichung, Taiwan

[6] Taipei Med Univ, Sch Med, Dept Surg, Coll Med, Taipei, Taiwan

[7] Bharathiar Univ, Dept Biotechnol, Coimbatore, Tamil Nadu, India

[8] China Med Univ, Dept Biol Sci & Technol, Taichung, Taiwan

[9] Ton Duc Thang Univ, Fac Sci Appl, Tan Phong Ward, Ho Chi Minh City, Vietnam

[10] Asia Univ, Dept Biol Sci & Technol, Taichung, Taiwan

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2018年 / 233卷 / 04期

关键词：

BCL2; Adenovirus E1B Nineteen kilo Dalton Interacting Protein 3 (BNIP3); diabetic hyperglycemic; forkhead box O3 (FoxO3); hyperglycemia; hypoxia-inducible factor-1 alpha (HIF-1); ischemia hypoxia; CARDIAC MYOCYTE DEATH; HYPOXIC ACCUMULATION; BNIP3; PROTEIN; PATHWAYS; STRESS; HIF-1-ALPHA; INHIBITION; INFARCTION; INDUCTION;

D O I：

10.1002/jcp.26235

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1 and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1 and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1 and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.

引用

页码：3660 / 3671

页数：12

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