Phytonutrient diet supplementation promotes beneficial Clostridia species and intestinal mucus secretion resulting in protection against enteric infection

被引:129
作者
Wlodarska, Marta [1 ,2 ]
Willing, Benjamin P. [4 ]
Bravo, David M. [5 ]
Finlay, B. Brett [1 ,2 ,3 ]
机构
[1] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada
[2] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z4, Canada
[3] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1Z4, Canada
[4] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada
[5] Pancosma, Geneva, Switzerland
基金
加拿大健康研究院;
关键词
ESCHERICHIA-COLI; LISTERIA-MONOCYTOGENES; AKKERMANSIA-MUCINIPHILA; ANTIMICROBIAL USE; MUCIN; EUGENOL; MICROBIOTA; GROWTH; MODEL; MECHANISMS;
D O I
10.1038/srep09253
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant extracts, or phytonutrients, are used in traditional medicine practices as supplements to enhance the immune system and gain resistance to various infectious diseases and are used in animal production as health promoting feed additives. To date, there are no studies that have assessed their mechanism of action and ability to alter mucosal immune responses in the intestine. We characterized the immunomodulatory function of six phytonutrients: anethol, carvacrol, cinnamaldehyde, eugenol, capsicum oleoresin and garlic extract. Mice were treated with each phytonutrient to assess changes to colonic gene expression and mucus production. All six phytonutrients showed variable changes in expression of innate immune genes in the colon. However only eugenol stimulated production of the inner mucus layer, a key mucosal barrier to microbes. The mechanism by which eugenol causes mucus layer thickening likely involves microbial stimulation as analysis of the intestinal microbiota composition showed eugenol treatment led to an increase in abundance of specific families within the Clostridiales order. Further, eugenol treatment confers colonization resistance to the enteric pathogen Citrobacter rodentium. These results suggest that eugenol acts to strengthen the mucosal barrier by increasing the thickness of the inner mucus layer, which protects against invading pathogens and disease.
引用
收藏
页数:9
相关论文
共 52 条
[1]   Modulation of intestinal goblet cell function during infection by an attaching and effacing bacterial pathogen [J].
Bergstrom, Kirk S. B. ;
Guttman, Julian A. ;
Rumi, Mohammad ;
Ma, Caixia ;
Bouzari, Saied ;
Khan, Mohammed A. ;
Gibson, Deanna L. ;
Vogl, A. Wayne ;
Vallance, Bruce A. .
INFECTION AND IMMUNITY, 2008, 76 (02) :796-811
[2]   Muc2 Protects against Lethal Infectious Colitis by Disassociating Pathogenic and Commensal Bacteria from the Colonic Mucosa [J].
Bergstrom, Kirk S. B. ;
Kissoon-Singh, Vanessa ;
Gibson, Deanna L. ;
Ma, Caixia ;
Montero, Marinieve ;
Sham, Ho Pan ;
Ryz, Natasha ;
Huang, Tina ;
Velcich, Anna ;
Finlay, B. Brett ;
Chadee, Kris ;
Vallance, Bruce A. .
PLOS PATHOGENS, 2010, 6 (05)
[3]  
Bosscher D, 2009, J PHYSIOL PHARMACOL, V60, P5
[4]   The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids [J].
Brown, AJ ;
Goldsworthy, SM ;
Barnes, AA ;
Eilert, MM ;
Tcheang, L ;
Daniels, D ;
Muir, AI ;
Wigglesworth, MJ ;
Kinghorn, I ;
Fraser, NJ ;
Pike, NB ;
Strum, JC ;
Steplewski, KM ;
Murdock, PR ;
Holder, JC ;
Marshall, FH ;
Szekeres, PG ;
Wilson, S ;
Ignar, DM ;
Foord, SM ;
Wise, A ;
Dowell, SJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (13) :11312-11319
[5]   The regulation of intestinal mucin MUC2 expression by short-chain fatty acids: implications for epithelial protection [J].
Burger-van Paassen, Nanda ;
Vincent, Audrey ;
Puiman, Patrycja J. ;
van der Sluis, Maria ;
Bouma, Janneke ;
Boehm, Gunther ;
van Goudoever, Johannes B. ;
Van Seuningen, Isabelle ;
Renes, Ingrid B. .
BIOCHEMICAL JOURNAL, 2009, 420 :211-219
[6]   Evaluation of carvacrol and eugenol as prophylaxis and treatment of vaginal candidiasis in an immunosuppressed rat model [J].
Chami, F ;
Chami, N ;
Bennis, S ;
Trouillas, J ;
Remmal, A .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (05) :909-914
[7]   Antibiotics in early life alter the murine colonic microbiome and adiposity [J].
Cho, Ilseung ;
Yamanishi, Shingo ;
Cox, Laura ;
Methe, Barbara A. ;
Zavadil, Jiri ;
Li, Kelvin ;
Gao, Zhan ;
Mahana, Douglas ;
Raju, Kartik ;
Teitler, Isabel ;
Li, Huilin ;
Alekseyenko, Alexander V. ;
Blaser, Martin J. .
NATURE, 2012, 488 (7413) :621-+
[8]   Gut Immune Maturation Depends on Colonization with a Host-Specific Microbiota [J].
Chung, Hachung ;
Pamp, Suenje J. ;
Hill, Jonathan A. ;
Surana, Neeraj K. ;
Edelman, Sanna M. ;
Troy, Erin B. ;
Reading, Nicola C. ;
Villablanca, Eduardo J. ;
Wang, Sen ;
Mora, Jorge R. ;
Umesaki, Yoshinori ;
Mathis, Diane ;
Benoist, Christophe ;
Relman, David A. ;
Kasper, Dennis L. .
CELL, 2012, 149 (07) :1578-1593
[9]   EXTENSION EDUCATION SYMPOSIUM: The future of biosecurity and antimicrobial use in livestock production in the United States and the role of extension [J].
Clark, S. ;
Daly, R. ;
Jordan, E. ;
Lee, J. ;
Mathew, A. ;
Ebner, P. .
JOURNAL OF ANIMAL SCIENCE, 2012, 90 (08) :2861-2872
[10]   Multifaceted transcriptional regulation of the murine intestinal mucus layer by endogenous microbiota [J].
Comelli, Elena M. ;
Simmering, Rainer ;
Faure, Magall ;
Donnicola, Dominique ;
Mansourian, Robert ;
Rochat, Florence ;
Corthesy-Theulaz, Irene ;
Cherbut, Christine .
GENOMICS, 2008, 91 (01) :70-77