Taxifolin and Sorghum Ethanol Extract Protect against Hepatic Insulin Resistance via the miR-195/IRS1/PI3K/AKT and AMPK Signalling Pathways

被引：16

作者：

Lee, Hana ^{[1
]}

Jeong, Won-Tae ^{[2
]}

So, Yoon-Sup ^{[3
]}

Lim, Heung-Bin ^{[4
]}

Lee, Junsoo ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Food Sci & Biotechnol, Cheongju 28644, South Korea

[2] Natl Inst Agr Sci, Residual Agrichem Assessment Div, Rural Dev Adm, Wonju 55365, South Korea

[3] Chungbuk Natl Univ, Dept Crop Sci, Cheongju 28644, South Korea

[4] Chungbuk Natl Univ, Dept Ind Plant Sci & Technol, Cheongju 28644, South Korea

来源：

ANTIOXIDANTS | 2021年 / 10卷 / 09期

关键词：

microRNA; insulin resistance; sorghum; taxifolin; miR-195; HEPG2; CELLS; ACTIVATION; MICRORNAS; GLUCOSE; TARGET; DIHYDROQUERCETIN; EPIGENETICS; EXPRESSION; PROFILES;

D O I：

10.3390/antiox10091331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to evaluate the effects of taxifolin and sorghum ethanol extract on free fatty acid (FFA)-induced hepatic insulin resistance. FFA treatment decreased glucose uptake by 16.2% compared with that in the control, whereas taxifolin and sorghum ethanol extract increased the glucose uptake. Additionally, taxifolin and sorghum ethanol extract increased the expression of p-PI3K, p-IRS1, p-AKT, p-AMPK, and p-ACC in FFA-induced hepatocytes. Furthermore, FFA treatment increased the expression of miR-195. However, compared with the FFA treatment, treatment with taxifolin and sorghum ethanol extract decreased miR-195 expression in a dose-dependent manner. Taxifolin and sorghum ethanol extract enhanced p-IRS1, p-PI3K, p-AMPK, p-AKT, and p-ACC expression by suppressing miR-195 levels in miR-195 mimic- or inhibitor-transfected cells. These results indicate that taxifolin and sorghum ethanol extract attenuate insulin resistance by regulating miR-195 expression, which suggests that taxifolin and sorghum ethanol extract may be useful antidiabetic agents.

引用

页数：13

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