Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination

被引：31

作者：

Dziarkowsk, Katarzyna ^{[2
]}

Jonsson, Jan Ake ^{[1
]}

Wieczorek, Piotr P. ^{[2
]}

机构：

[1] Lund Univ, Dept Analyt Chem, S-22100 Lund, Sweden

[2] Opole Univ, Inst Chem, PL-45052 Opole, Poland

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 606卷 / 02期

关键词：

polyamines; supported liquid membrane; hollow fiber membrane; extraction technique;

D O I：

10.1016/j.aca.2007.11.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization. procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyarnines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：184 / 193

页数：10

共 31 条

[1] Supported liquid membrane work-up of blood plasma samples coupled on-line to liquid chromatographic determination of basic antidepressant drugs [J].