Effect of electron-transport inhibitors on the generation of reactive oxygen species by pea mitochondria during succinate oxidation

被引：9
作者：
Popov, VN
Ruuge, EK
Starkov, AA
机构：
[1] Voronezh State Univ, Dept Plant Physiol & Biochem, Voronezh 394693, Russia
[2] Minist Publ Hlth Russia, Russian Cardiol Res & Prod Assoc, Moscow 121552, Russia
[3] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119992, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2003年 / 68卷 / 07期
基金：
俄罗斯基础研究基金会;
关键词：
reactive oxygen species; mitochondria; electron transport; alternative oxidase; myxothiazol; antimycin A; cyanide;
D O I：
10.1023/A:1025078815819
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of inhibitors of the cytochrome pathway and alternative oxidase on the rate of respiration and generation of reactive oxygen species by pea mitochondria was studied. Respiration of mitochondria from pea cotyledons was inhibited by 70-80% by salicylhydroxamate (SHAM). The rate of hydrogen peroxide production by pea cotyledon mitochondria during succinate oxidation was 0.15 nmol/min per mg protein. SHAM considerably accelerated the hydrogen peroxide production. The SHAM-dependent H2O2 production was stimulated by 2 muM antimycin A and inhibited by 5 mM KCN and 1 muM myxothiazol. The study of the rate of O-2(radical anion) generation by pea mitochondria using EPR spin traps and epinephrine oxidation showed that H2O2 accumulation can be accounted for by a significant increase in the rate of O-2(radical anion) production.
引用
收藏
页码：747 / 751
页数：5
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