In this chapter, we discuss the need for the development of enzyme-activatable probes in the field of tumor-targeted photoacoustic (PA) imaging, then we give a brief description of the innovation of designing alkaline phosphatase (ALP)-activatable probes for PA imaging. After that, we provide detailed protocols for the syntheses and characterizations of a near-infrared photoacoustic imaging probe, 1P, developed in our research group. With this tool, 1P could form nanoparticles 1-NPs under the catalysis of ALP and thus could be used to enhance PA imaging both in vitro and in vivo.
机构:
Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48019 USA
Natl Ctr Nanosci & Technol, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Ctr Excellence Nanosci, Beijing 100049, Peoples R China
Chinese Acad Sci, Changchun Inst Appl Chem, Biol Chem Lab, Changchun 130022, Jilin, Peoples R ChinaUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Yan, Jiao
Fang, Wenchun
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US FDA, Silver Spring, MD 20993 USAUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Fang, Wenchun
Kim, Ji-Young
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Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48019 USAUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Kim, Ji-Young
Lu, Jun
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Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48019 USAUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Lu, Jun
Kumar, Prashant
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Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48019 USAUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Kumar, Prashant
Mu, Zhengzhi
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Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48019 USA
Jilin Univ, Key Lab Bion Engn, Minist Educ, Changchun 130022, Peoples R ChinaUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Mu, Zhengzhi
Wu, Xiaochun
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Natl Ctr Nanosci & Technol, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Ctr Excellence Nanosci, Beijing 100049, Peoples R ChinaUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Wu, Xiaochun
Mao, Xiaoming
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Univ Michigan, Dept Phys, Ann Arbor, MI 48019 USAUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Mao, Xiaoming
Kotov, Nicholas A.
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Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA
Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48019 USAUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48019 USA