Vertically aligned carbon nanofiber electrode arrays for nucleic acid detection

被引：19

作者：

Arumugam, Prabhu U. ^{[1
]}

Yu, Edmond ^{[1
]}

Riviere, Roger ^{[1
]}

Meyyappan, M. ^{[1
]}

机构：

[1] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA

来源：

CHEMICAL PHYSICS LETTERS | 2010年 / 499卷 / 4-6期

关键词：

FABRICATION;

D O I：

10.1016/j.cplett.2010.09.048

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We present electrochemical detection of DNA targets that corresponds to Escherichia coli O157:H7 16S rRNA gene using a nanoelectrode array consisting of vertically aligned carbon nanofiber (VACNF) electrodes. Parylene C is used as gap filling 'matrix' material to avoid high temperature processing in electrode construction. This easy to deposit film of several micron heights provides a conformal coating between the high aspect ratio VACNFs with negligible pin-holes. The low background currents show the potential of this approach for ultra-sensitive detection. Consistent and reproducible electrochemical-signals are achieved using a simple electrode preparation. This simple, reliable and low-cost approach is a forward step in developing practical sensors for applications like pathogen detection, early cancer diagnosis and environmental monitoring. (C) 2010 Elsevier B.V. All rights reserved.

引用

收藏

页码：241 / 246

页数：6

相关论文

共 19 条

[1] Dielectrophoretic trapping of single bacteria at carbon nanofiber nanoelectrode arrays [J].

Arumugam, Prabhu U. ;

Chen, Hua ;

Cassell, Alan M. ;

Li, Jun .

JOURNAL OF PHYSICAL CHEMISTRY A, 2007, 111 (49) :12772-12777

[2] Wafer-scale fabrication of patterned carbon nanofiber nanoelectrode arrays: A route for development of multiplexed, ultrasensitive disposable biosensors [J].

Arumugam, Prabhu U. ;

Chen, Hua ;

Siddiqui, Shabnam ;

Weinrich, Jarret A. P. ;

Jejelowo, Ayodeji ;

Li, Jun ;

Meyyappan, M. .

BIOSENSORS & BIOELECTRONICS, 2009, 24 (09) :2818-2824

[3] Fabrication and characterization of vertically aligned carbon nanofiber electrodes for biosensing applications [J].

Baker, SE ;

Tse, KY ;

Lee, CS ;

Hamers, RJ .

DIAMOND AND RELATED MATERIALS, 2006, 15 (2-3) :433-439

[4]

Bard A.J., 2001, ELECTROCHEMICAL METH, VSecond, P4

[5] Developing nucleic acid-based electrical detection systems [J].

Gabig-Ciminska, M .

MICROBIAL CELL FACTORIES, 2006, 5 (1)

[6] Individually addressable vertically aligned carbon nanofiber-based electrochemical probes [J].

Guillorn, MA ;

McKnight, TE ;

Melechko, A ;

Merkulov, VI ;

Britt, PF ;

Austin, DW ;

Lowndes, DH ;

Simpson, ML .

JOURNAL OF APPLIED PHYSICS, 2002, 91 (06) :3824-3828

[7] A versatile microreactor platform featuring a chemical-resistant microvalve array for addressable multiplex syntheses and assays [J].

Hua, Zhishan ;

Xia, Yongmei ;

Srivannavit, Onnop ;

Rouillard, Jean-Marie ;

Zhou, Xiaochuan ;

Gao, Xiaolian ;

Gulari, Erdogan .

JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2006, 16 (08) :1433-1443

[8] ELECTROCHEMICAL MEASUREMENT OF THE SOLVENT ACCESSIBILITY OF NUCLEOBASES USING ELECTRON-TRANSFER BETWEEN DNA AND METAL-COMPLEXES [J].

JOHNSTON, DH ;

GLASGOW, KC ;

THORP, HH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (35) :8933-8938

[9]

JUNGKYU K, 2010, P SOC PHOTO-OPT INS, V7593, P955

[10] Ultrasensitive label-free DNA analysis using an electronic chip based on carbon nanotube nanoelectrode arrays [J].

Koehne, J ;

Chen, H ;

Li, J ;

Cassell, AM ;

Ye, Q ;

Ng, HT ;

Han, J ;

Meyyappan, M .

NANOTECHNOLOGY, 2003, 14 (12) :1239-1245