Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production

被引：44

作者：

Sanchez, Elena G. ^{[1
]}

Riera, Elena ^{[1
]}

Nogal, Marisa ^{[1
]}

Gallardo, Carmina ^{[5
]}

Fernandez, Paloma ^{[1
]}

Bello-Morales, Raquel ^{[3
]}

Antonio Lopez-Guerrero, Jose ^{[3
]}

Chitko-McKown, Carol G. ^{[4
]}

Richt, Jurgen A. ^{[2
]}

Revilla, Yolanda ^{[1
]}

机构：

[1] UAM, CSIC, Ctr Biol Mol Severo Ochoa, Virol Dept, Madrid, Spain

[2] Kansas State Univ, Coll Vet Med, CEEZAD, Manhattan, KS 66506 USA

[3] Univ Autonoma Madrid, Mol Biol Dept, Madrid, Spain

[4] USDA ARS, USMARC, Clay Ctr, POB 166, Clay Ctr, NE 68933 USA

[5] INIA, CISA, European Union Reference Lab ASF, Madrid, Spain

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

MYELOMONOCYTIC MARKERS; MONOCLONAL-ANTIBODIES; EXPRESSION; MACROPHAGES; ISOLATE; CD163; GENERATION; ANTIGEN; ENTRY; PIGS;

D O I：

10.1038/s41598-017-09948-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, C Delta 2+ and WSL, were compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibility to ASFV infection and virus production. The virulent ASFV Armenia/07, E70 or the naturally attenuated NHV/P68 strains were used as viral models. Cells expressed only low levels of specific receptors linked to the monocyte/macrophage lineage, with low levels of infection overall, with the exception of WSL, which showed more efficient production of strain NHV/P68 but not of strains E70 and Armenia/07.

引用

页数：13

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