Determination of scopoletin in rat plasma by high performance liquid chromatographic method with UV detection and its application to a pharmacokinetic study

被引：32
作者：
Xia, Yufeng [1 ]
Dai, Yue
Wang, Qiang
Liang, Huizheng
机构：
[1] China Pharmaceut Univ, Dept Chinese Mat Med Anal, Nanjing 210038, Peoples R China
[2] Jiangsu Key Lab Mol & Med Biotechnol, Nanjing 210046, Peoples R China
[3] China Pharmaceut Univ, Dept Pharmacol Chinese Mat Med, Nanjing 210038, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 857卷 / 02期
关键词：
scopoletin; HPLC; plasma; pharmacokinetics;
D O I：
10.1016/j.jchromb.2007.07.023
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and simple high-performance liquid chromatographic (HPLC) method has been developed and validated for determination of scopoletin in rat plasma using psoralen as internal standard. Chromatographic separation was achieved on a C-18 column using methanol and distilled water (49:51, v/v) containing 0.05% (v/v) phosphoric acid as mobile phase. The UV detector was set at 345 nm. The calibration curve was linear over the range of 0.165-9.90 mu g/ml with a correlation coefficient of 0.9994. The recovery for plasma samples of 0.165, 1.32 and 6.60 mu g/ml was 93.2%, 95.9% and 95.5%, respectively. The RSD of intra- and inter-day assay variations was less than 6.7%. This HPLC assay is a precise and reliable method for the analysis of scopoletin in pharmacokinetic studies. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：332 / 336
页数：5
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