Evaluation of fatty acid profiles of Chlorella Vulgaris microalgae grown in dairy wastewater for producing biofuel

Biodiesel is a biofuel made from plant oils and animal lipids. Utilization of lipid accumulation in algae biomass as biodiesel is a good alternative to fossil fuels. In this research Chlorella vulgaris microalga was applied after planting in BG11 culture medium in effluent and wastewater of dairy industry after preparation of 25, 50, and 75% dilutions. Algae in two concentrations with low (13 million cells/mL) and high density (26 million cells/mL) were injected. According to the results obtained in the wastewater environment the highest amount of C16:0 fatty acid was observed in F2 25% treatment, and C18:0 fatty acid is related to F1 75% treatment. In the effluent environment, the highest amount of fatty acids C16:0 and C16:1n7 occur in P1 50% treatment, and C18:0 and C18:3n3 fatty acids are related to P1 50% treatment, respectively. The highest amount of saturated fatty acids (SFA) was reported at P2 75% treatment (56.25%) and monounsaturated fatty acids (MUFA) has accumulated in F175% (40.13%) treatment. Chlorella vulgaris microalgae can be considered as a rich source of lipid and fatty acid profiles in both wastewater and effluents, and it can be regarded as potential significance source for biodiesel production.