Phylogenetic grouping by pcr analyses of Sinorhizobium meliloti strains isolated from eutrophic soil

被引：0

作者：

Oliveira, WS ^{[1
]}

Oliveira, PPA ^{[1
]}

Bellato, CM ^{[1
]}

Duarte, FRS ^{[1
]}

Tsai, SM ^{[1
]}

机构：

[1] Univ Sao Paulo, CENA, Piracicaba, SP, Brazil

来源：

PROCEEDINGS OF THE XIX INTERNATIONAL GRASSLAND CONGRESS: GRASSLAND ECOSYSTEMS: AN OUTLOOK INTO THE 21ST CENTURY | 2001年

关键词：

alfalfa; phylogenetic grouping; Sinorhizobium meliloti;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study evaluated the ability of Sinorhizobium meliloti strains (SEMIA-116, SEMIA-134 and SEMIA-135) to persist in eutrophic soils after three complete growing cycles of alfalfa. PCR fingerprinting using primers for the nifH, Eric, Rep, RP genes and P25 RAPD-PCR performed the phylogenetic grouping of the three commercial strains and 39 field isolates. These analyzes were sufficiently sensitive to discriminate and group the strains and isolate. Our preliminary studies were based upon Eric primers and RP01 RAPD-PCR.

引用

页码：591 / 592

页数：2

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