Enhanced biodegradation of pyrene by immobilized bacteria on modified biomass materials

被引：31

作者：

Deng, Fucai ^{[1
]}

Liao, Changjun ^{[1
,2
]}

Yang, Chen ^{[1
,3
]}

Guo, Chuling ^{[1
,3
]}

Dang, Zhi ^{[1
,3
]}

机构：

[1] S China Univ Technol, Coll Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangdong Vocat Coll Environm Protect Engn, Dept Environm Engn, Foshan 528216, Peoples R China

[3] Minist Educ, Key Lab Pollut Control & Ecosyst Restorat Ind Clu, Beijing, Peoples R China

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2016年 / 110卷

基金：

国家高技术研究发展计划(863计划);

关键词：

Pyrene; Biodegradation; Bacteria immobilization; Biomass; Chemical modification; C-13; NMR; PHENANTHRENE BIODEGRADATION; CALCIUM-ALGINATE; DEGRADATION; ADSORPTION; WATER; BIOREMEDIATION; EQUILIBRIUM; LIGNIN; CELLS; STRAW;

D O I：

10.1016/j.ibiod.2016.02.016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The degradation of pyrene (PYR) by Mycobacterium gilvum immobilized on peanut shell powder (PSP) was improved by chemically modifying the PSP. The physicochemical properties of the modified biomass (M-PSP) were characterized with surface area analysis, X-ray diffraction, Fourier transform infrared and solid-state CP/MAS C-13 NMR spectra. The results showed that the chemical modification decreased the crystallinity of the biomass and destroyed the benzene rings in the cellulose, which improved the porosity of PSP. Fluorescein diacetate activity (FDA) of cells immobilized on the M-PSP decreased more slowly than cells immobilized on PSP. The PYR degradation efficiencies achieved by bacteria immobilized on M-PSP was higher than that achieved by immobilized bacteria on PSP after 7 d. It revealed that chemical modification of biomass could further improve bacterial activity and enhance the degradation ability of the cells compared with the raw one, as we expected. In summary, this study shows that M-PSP is a good immobilizing bio-mass for supporting pollutant degrading bacteria and could be employed as an effective material for PYR biodegradation. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：46 / 52

页数：7

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