Rapid nucleic acid detection of Zaire ebolavirus on paper fluidics

被引:9
作者
Fang, Xueen [1 ,2 ,3 ]
Guan, Ming [1 ,2 ]
Kong, Jilie [1 ,2 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Fudan Univ, Huashan Hosp, Shanghai 200433, Peoples R China
[3] Shanghai Suxin Biotech Co Ltd, Shanghai 200433, Peoples R China
基金
中国国家自然科学基金;
关键词
MEDIATED ISOTHERMAL AMPLIFICATION; DEVICES; VIRUSES;
D O I
10.1039/c5ra09430e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We described a novel system of paper fluidics, in which isothermal nucleic acid amplification and detection are performed. This paper system made the nucleic acid test simpler, easier to operate, cheap, and provided the performance of point-of-care testing. This system was used to detect the ebolavirus rapidly with high specificity and sensitivity.
引用
收藏
页码:64614 / 64616
页数:3
相关论文
共 21 条
[1]   Ebolavirus VP35 Coats the Backbone of Double-Stranded RNA for Interferon Antagonism [J].
Bale, Shridhar ;
Julien, Jean-Philippe ;
Bornholdt, Zachary A. ;
Krois, Alexander S. ;
Wilson, Ian A. ;
Saphire, Erica Ollmann .
JOURNAL OF VIROLOGY, 2013, 87 (18) :10385-10388
[2]   Laminated paper-based analytical devices (LPAD): fabrication, characterization, and assays [J].
Cassano, Christopher L. ;
Fan, Z. Hugh .
MICROFLUIDICS AND NANOFLUIDICS, 2013, 15 (02) :173-181
[3]   Paper-based microfluidics with high resolution, cut on a glass fiber membrane for bioassays [J].
Fang, Xueen ;
Wei, Shasha ;
Kong, Jilie .
LAB ON A CHIP, 2014, 14 (05) :911-915
[4]   Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics [J].
Foudeh, Amir M. ;
Didar, Tohid Fatanat ;
Veres, Teodor ;
Tabrizian, Maryam .
LAB ON A CHIP, 2012, 12 (18) :3249-3266
[5]   Nucleic acid isothermal amplification technologies - A review [J].
Gill, Pooria ;
Ghaemi, Amir .
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2008, 27 (03) :224-243
[6]   Associations between DNA methylation and schizophrenia-related intermediate phenotypes - A gene set enrichment analysis [J].
Hass, Johanna ;
Walton, Esther ;
Wright, Carrie ;
Beyer, Andreas ;
Scholz, Markus ;
Turner, Jessica ;
Liu, Jingyu ;
Smolka, Michael N. ;
Roessner, Veit ;
Sponheim, Scott R. ;
Gollub, Randy L. ;
Calhoun, Vince D. ;
Ehrlich, Stefan .
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, 2015, 59 :31-39
[7]   Determination of Specific Antibody Responses to the Six Species of Ebola and Marburg Viruses by Multiplexed Protein Microarrays [J].
Kamata, Teddy ;
Natesan, Mohan ;
Warfield, Kelly ;
Aman, M. Javad ;
Ulrich, Robert G. .
CLINICAL AND VACCINE IMMUNOLOGY, 2014, 21 (12) :1605-1612
[8]   Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification [J].
Kurosaki, Yohei ;
Takada, Ayato ;
Ebihara, Hideki ;
Grolla, Allen ;
Kamo, Naoki ;
Feldmann, Heinz ;
Kawacka, Yoshihiro ;
Yasuda, Jiro .
JOURNAL OF VIROLOGICAL METHODS, 2007, 141 (01) :78-83
[9]   Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of herpes simplex virus type 2-specific immunoglobulin G antibodies in serum and whole blood [J].
Laderman, Elisabeth I. ;
Whitworth, Emma ;
Dumaual, Erickson ;
Jones, Mark ;
Hudak, Andrew ;
Hogrefe, Wayne ;
Carney, Jim ;
Groen, Jan .
CLINICAL AND VACCINE IMMUNOLOGY, 2008, 15 (01) :159-163
[10]   Three-dimensional microfluidic devices fabricated in layered paper and tape [J].
Martinez, Andres W. ;
Phillips, Scott T. ;
Whitesides, George M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (50) :19606-19611