Local lymph node activation and IgE responses in Brown Norway and Wistar rats after dermal application of sensitizing and non-sensitizing chemicals

被引:46
作者
Arts, JHE [1 ]
Droge, SCM [1 ]
Spanhaak, S [1 ]
Bloksma, N [1 ]
Penninks, AH [1 ]
Kuper, CF [1 ]
机构
[1] UNIV UTRECHT,RITOX,TOXICOL RES INST,UTRECHT,NETHERLANDS
关键词
IgE; lymph node activation; rats; dermal application; sensitizers;
D O I
10.1016/S0300-483X(96)03576-7
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The local lymph node assay (LLNA) and the IgE test in the mouse are proposed models for predictive recognition of low molecular weight chemicals causing IEE-mediated allergic airway reactions in man. Since rats are commonly used in routine toxicity studies and a previous study (Arts et al. (1996) Food Chem. Toxicol. 34, 55-62) has shown that several rat strains were found appropriate for the LLNA, the suitability of the rat for the IgE test was examined in the present study. Serum IgE concentrations were examined following topical exposure of Brown Norway (BN) and Wistar rats to each of four chemicals with known diverse sensitization potential in humans: trimellitic anhydride (TMA), a dermal and respiratory sensitizer; dinitrochlorobenzene (DNCB), a dermal sensitizer with no or limited potential to cause respiratory allergy; formaldehyde (FA), a skin irritant and dermal sensitizer with equivocal evidence for respiratory sensitizing potential; methyl salicylate (MS), a skin irritant devoid of sensitizing properties. Of the four tested chemicals, only exposure to TMA resulted in a significant increase in serum IgE concentration and this response was only evoked in the high-IgE-responding BN rat. The latter two chemicals were also tested for lymph node activation, in casu the ear-draining lymph nodes. FA caused a dose-dependent activation of the draining lymph nodes whereas MS was inactive. The results as obtained with TMA, DNCB and MS in the rat are in agreement with human data. The results with FA though, indicate the need for further studies of chemicals that have both irritant and sensitizing properties at about similar concentrations or may act through non-IgE-mediated immune mechanisms. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:229 / 237
页数:9
相关论文
共 44 条
  • [1] ABADIE A, 1980, IMMUNOLOGY, V39, P561
  • [2] ATOPY AND BRONCHIAL HYPERRESPONSIVENESS - EXCLUSION OF LINKAGE TO MARKERS ON CHROMOSOME-11Q AND CHROMOSOME-6P
    AMELUNG, PJ
    PANHUYSEN, CIM
    POSTMA, DS
    LEVITT, RC
    KOETER, GH
    FRANCOMANO, CA
    BLEECKER, ER
    MEYERS, DA
    [J]. CLINICAL AND EXPERIMENTAL ALLERGY, 1992, 22 (12) : 1077 - 1084
  • [3] Local lymph node activation in rats after dermal application of the sensitizers 2,4-dinitrochlorobenzene and trimellitic anhydride
    Arts, JHE
    Droge, SCM
    Bloksma, N
    Kuper, CF
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 1996, 34 (01) : 55 - 62
  • [4] THE PERFORMANCE OF THE LOCAL LYMPH-NODE ASSAY WITH CHEMICALS IDENTIFIED AS CONTACT ALLERGENS IN THE HUMAN MAXIMIZATION TEST
    BASKETTER, DA
    SCHOLES, EW
    KIMBER, I
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 1994, 32 (06) : 543 - 547
  • [5] THE RELATIONSHIP OF AIRBORNE TRIMELLITIC ANHYDRIDE CONCENTRATIONS TO TRIMELLITIC ANHYDRIDE INDUCED SYMPTOMS AND IMMUNE-RESPONSES
    BERNSTEIN, DI
    ROACH, DE
    MCGRATH, KG
    LARSEN, RS
    ZEISS, CR
    PATTERSON, R
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1983, 72 (06) : 709 - 713
  • [6] A 2-CENTER STUDY FOR THE EVALUATION AND VALIDATION OF AN ANIMAL-MODEL FOR THE ASSESSMENT OF THE POTENTIAL OF SMALL MOLECULAR-WEIGHT CHEMICALS TO CAUSE RESPIRATORY ALLERGY
    BLAIKIE, L
    MORROW, T
    WILSON, AP
    HEXT, P
    HARTOP, PJ
    RATTRAY, NJ
    WOODCOCK, D
    BOTHAM, PA
    [J]. TOXICOLOGY, 1995, 96 (01) : 37 - 50
  • [7] PREDICTIVE IMMUNOTOXICOLOGICAL TEST SYSTEMS - SUITABILITY OF THE POPLITEAL LYMPH-NODE ASSAY IN MICE AND RATS
    BLOKSMA, N
    KUBICKAMURANYI, M
    SCHUPPE, HC
    GLEICHMANN, E
    GLEICHMANN, H
    [J]. CRITICAL REVIEWS IN TOXICOLOGY, 1995, 25 (05) : 369 - 396
  • [8] BLOKSMA N, 1993, PHARM TOXICOL S2, V73, P34
  • [9] SENSITIZATION OF GUINEA-PIGS BY INHALATION EXPOSURE TO LOW-MOLECULAR WEIGHT CHEMICALS
    BOTHAM, PA
    HEXT, PM
    RATTRAY, NJ
    WALSH, ST
    WOODCOCK, DR
    [J]. TOXICOLOGY LETTERS, 1988, 41 (02) : 159 - 173
  • [10] THE INDUCTION OF RESPIRATORY ALLERGY IN GUINEA-PIGS FOLLOWING INTRADERMAL INJECTION OF TRIMELLITIC ANHYDRIDE - A COMPARISON WITH THE RESPONSE TO 2,4-DINITROCHLOROBENZENE
    BOTHAM, PA
    RATTRAY, NJ
    WOODCOCK, DR
    WALSH, ST
    HEXT, PM
    [J]. TOXICOLOGY LETTERS, 1989, 47 (01) : 25 - 39