An Electrospun Sandwich-Type Lipase-Membrane Bioreactor for Hydrolysis at Macroscopic Oil-Water Interfaces

被引：12
作者：
Kuang, Lei [1 ,2 ]
Zhang, Qianqian [1 ,2 ]
Li, Jinlong [1 ,2 ]
Tian, Huafeng [3 ]
机构：
[1] Beijing Technol & Business Univ, Sch Food & Hlth, Beijing 100048, Peoples R China
[2] Beijing Technol & Business Univ, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China
[3] Beijing Technol & Business Univ, Sch Chem & Mat Engn, Beijing 100048, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2022年 / 70卷 / 02期
基金：
中国国家自然科学基金;
关键词：
lipase; electrospinning; membrane bioreactor; macroscopic interface; MICROBIAL LIPASES; OLIVE OIL; IMMOBILIZATION; PERFORMANCE; ACTIVATION; NANOFIBERS; DESIGN;
D O I：
10.1021/acs.jafc.1c04042
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The core task for lipase catalytic system design is to construct a suitable oil-water interface for lipase distribution. In comparison to the micro-oil-water interface, the macro-oil-water interface (top oil-bottom water) served as a simplified lipase catalytic system that is more in line with industrial applications but limited in catalytic efficiency. Based on the assumption that one potential carrier can help lipase reach to the macro-oil-water interface, in the current work, sandwich-type lipase-membrane bioreactors (SLMBs) fabricated by a facile layer-by-layer electrospinning process were reported. These SLMBs were composed of a hydrophilic polyamide 6 nanofibrous membrane (NFM) as the bottom layer, a blended electrospun lipase/PVA NFM as the middle layer, and a hydrophobic EC/PU NFM as the top layer. The lipase loading can be controlled by altering the electrospinning time of the middle layer. Under the optimized conditions, the catalytic efficiency of the SLMBs was 2.05 times higher than that of free lipase. In addition, the SLMBs exhibit much better pH (high activity over a broad pH range of 5-10), temperature (retained 62% at 80 degrees C), storage stability (no loss of activity after being stored at 4 degrees C for 11 days), and reusability (retained 23% after five cycles) than free lipase.
引用
收藏
页码：584 / 591
页数：8
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