Docosahexaenoic Acid Alleviates Palmitic Acid-Induced Inflammation of Macrophages via TLR22-MAPK-PPARγ/Nrf2 Pathway in Large Yellow Croaker (Larimichthys crocea)

被引：16

作者：

Xu, Dan ^{[1
]}

Cui, Kun ^{[1
]}

Li, Qingfei ^{[1
]}

Zhu, Si ^{[1
]}

Zhang, Junzhi ^{[1
]}

Gao, Shengnan ^{[1
]}

Hao, Tingting ^{[1
]}

Mai, Kangsen ^{[1
,2
]}

Ai, Qinghui ^{[1
,2
]}

机构：

[1] Ocean Univ China, Key Lab Aquaculture Nutr & Feed, Minist Agr & Rural Affairs, Key Lab Mariculture,Minist Educ, 5 Yushan Rd, Qingdao 266003, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Peoples R China

来源：

ANTIOXIDANTS | 2022年 / 11卷 / 04期

基金：

中国国家自然科学基金;

关键词：

palmitic acid; inflammatory response; Toll-like receptor; docosahexaenoic acid; Larimichthys crocea; POLYUNSATURATED FATTY-ACIDS; NF-KAPPA-B; RECEPTOR; 22; TLR22; MOLECULAR CHARACTERIZATION; ADIPOSE-TISSUE; PPAR-GAMMA; FISH-OIL; EXPRESSION; IMMUNITY; STRESS;

D O I：

10.3390/antiox11040682

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Palmitic acid (PA) is a saturated fatty acid (SFA) that can cause an inflammatory response, while docosahexaenoic acid (DHA) is always used as a nutritional modulator due to its anti-inflammatory properties. However, the potential molecular mechanism is still not completely elucidated in fish. Herein, the PA treatment induced an inflammatory response in macrophages of large yellow croaker (Larimichthys crocea). Meanwhile, the mRNA expression of Toll-like receptor (TLR)-related genes, especially tlr22, and the phosphorylation of the mitogen-activated protein kinase (MAPK) pathway were significantly upregulated by PA. Further investigation found that the PA-induced inflammatory response was suppressed by tlr22 knockdown and MAPK inhibitors. Moreover, the results of the peroxisome proliferator-activated receptor gamma (PPAR gamma) agonist and inhibitor treatment proved that PPAR gamma was involved in the PA-induced inflammation. PA treatment decreased the protein expression of PPAR gamma, while tlr22 knockdown and MAPK inhibitors recovered the decreased expression. Besides, the PA-induced activation of Nrf2 was regulated by p38 MAPK. Furthermore, DHA-executed anti-inflammatory effects by regulating the phosphorylation of the MAPK pathway and expressions of PPAR gamma and Nrf2. Overall, the present study revealed that DHA alleviated PA-induced inflammation in macrophages via the TLR22-MAPK-PPAR gamma/Nrf2 pathway. These results could advance the understanding of the molecular mechanism of the SFA-induced inflammatory response and provide nutritional mitigative strategies.

引用

页数：15

共 54 条

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