Horizontal gene transfer rate is not the primary determinant of observed antibiotic resistance frequencies in Streptococcus pneumoniae

被引：15

作者：

Lehtinen, Sonja ^{[1
,2
]}

Chewapreecha, Claire ^{[3
,4
,5
]}

Lees, John ^{[6
]}

Hanage, William P. ^{[7
]}

Lipsitch, Marc ^{[7
]}

Croucher, Nicholas J. ^{[7
]}

Bentley, Stephen D. ^{[3
]}

Turner, Paul ^{[8
,9
]}

Fraser, Christophe ^{[1
]}

Mostowy, Rafal J. ^{[1
,6
,10
]}

机构：

[1] Univ Oxford, Big Data Inst, Oxford, England

[2] Swiss Fed Inst Technol, Dept Environm Syst Sci, Inst Integrat Biol, Zurich, Switzerland

[3] Wellcome Sanger Inst, Hinxton, England

[4] Mahidol Oxford Trop Med Res Unit, Bangkok, Thailand

[5] King Mongkuts Univ Technol Thonburi, Sch Bioresource & Technol, Bioinformat & Syst Biol Program, Bangkok, Thailand

[6] Imperial Coll London, Sch Publ Hlth, Dept Infect Dis Epidemiol, London, England

[7] Harvard Univ, Ctr Communicable Dis Dynam, Dept Epidemiol, Harvard TH Chan Sch Publ Hlth, Boston, MA 02115 USA

[8] Angkor Hosp Children, Cambodia Oxford Med Res Unit, Siem Reap, Cambodia

[9] Univ Oxford, Ctr Trop Med & Global Hlth, Oxford, England

[10] Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, Poland

来源：

SCIENCE ADVANCES | 2020年 / 6卷 / 21期

基金：

英国惠康基金;

关键词：

EVOLUTION; RECOMBINATION;

D O I：

10.1126/sciadv.aaz6137

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The extent to which evolution is constrained by the rate at which horizontal gene transfer (HGT) allows DNA to move between genetic lineages is an open question, which we address in the context of antibiotic resistance in Streptococcus pneumoniae. We analyze microbiological, genomic, and epidemiological data from the largest-to-date sequenced pneumococcal carriage study in 955 infants from a refugee camp on the Thailand-Myanmar border. Using a unified framework, we simultaneously test prior hypotheses on rates of HGT and a key evolutionary covariate (duration of carriage) as determinants of resistance frequencies. We conclude that in this setting, there is little evidence of HGT playing a major role in determining resistance frequencies. Instead, observed resistance frequencies are best explained as the outcome of selection acting on a pool of variants, irrespective of the rate at which resistance determinants move between genetic lineages.

引用

页数：8

共 29 条

[11] International genomic definition of pneumococcal lineages, to contextualise disease, antibiotic resistance and vaccine impact [J].