Label-free photoelectrochemical immunoassay for CEA detection based on CdS sensitized WO3@BiOI heterostructure nanocomposite

被引：218

作者：

Han, Qingzhi ^{[1
]}

Wang, Rongyu ^{[1
]}

Xing, Bin ^{[1
]}

Zhang, Tong ^{[1
]}

Khan, Malik Saddam ^{[1
]}

Wu, Dan ^{[1
]}

Wei, Qin ^{[1
]}

机构：

[1] Univ Jinan, Key Lab Interfacial React & Sensing Anal Univ Sha, Sch Chem & Chem Engn, Jinan 250022, Shandong, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2018年 / 99卷

基金：

中国国家自然科学基金;

关键词：

Label-free immunosensor; Photoelectrochemistry; CEA; WO3@BiOI@CdS hybrid structure; VISIBLE-LIGHT; PHOTOCATALYTIC ACTIVITY; QUANTUM DOTS; CARCINOEMBRYONIC ANTIGEN; PROGNOSTIC-FACTORS; TIO2; NANOTUBES; SERUM CEA; NANOPARTICLES; GRAPHENE; CANCER;

D O I：

10.1016/j.bios.2017.08.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work, a label-free photoelectrochemical (PEC) immunosensor for the detection of carcino embryonic antigen (CEA) was developed based on CdS nanowires (NWs) sensitized WO3@BiOI heterostructure. The construction procedure of ITO/WO3@BIOI@CdS electrode was done by dipping the WO3@BiOI modified indium-tin oxide (ITO) electrode interchangeably into [Cd(NH3)(4)](2+) and S2- solution for several times. Then the ITO/WO3@BiOI@CdS electrode was used as a matrix for the subsequent immobilization of CEA antibody (Ab). The obtained label-free PEC immunosensor showed an excellent PEC performance toward CEA detection. Under optimal conditions, the PEC immunosensor have a sensitive response to CEA in a liner range of 0.01 ng/ mL to 50 ng/mL with a detection limit of 3.2 pg/mL. The proposed PEC immunosensor exhibited good stability, high sensitivity as well as reproducibility and storage stability. Moreover, the obtained PEC immunosensor also showed a satisfied result in human serum sample analysis.

引用

页码：493 / 499

页数：7

共 45 条

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