Identification of the 5′ terminal sequence of the SAR-55 and MEX-14 strains of hepatitis E virus and confirmation that the genome is capped

被引：35

作者：

Zhang, MD

Purcell, RH

Emerson, SU ^{[1
]}

机构：

[1] NIAID, Hepatitis Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

[2] NIAID, Mol Hepatitis Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF MEDICAL VIROLOGY | 2001年 / 65卷 / 02期

关键词：

cap-dependent RACE; conserved; 5; termini; Burmese strain;

D O I：

10.1002/jmv.2032

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis E virus (HEV) is a nonenveloped virus with a genome of sing le-stranded, positive-sense RNA. The 5 ' terminal sequence of two HEV strains (SAR-55 and MEX-14) was determined by a 5 ' RNA ligase-mediated rapid amplification of cDNA ends (RACE) method designed to select capped RNAs. The 5 ' noncoding region of the SAR-55 and MEX-14 strains were amplified, confirming that the genomic RNA of HEV is capped. The 5 ' noncoding region of the SAR-55 strain had 25 nucleotides, which is two less than reported for the Burmese strain, and that of the MEX-14 strain had 24 nucleotides, which is 21 more than reported previously [Huang et al., 1992]. (C) 2001 Wiley-Liss, Inc.

引用

页码：293 / 295

页数：3

共 12 条

[1]

Donati MC, 1997, VIRAL HEPATITIS LIVE, P313

[2] MOLECULAR-CLONING AND SEQUENCING OF THE MEXICO ISOLATE OF HEPATITIS-E VIRUS (HEV) [J].