The use of the antimicrobial peptide piscidin (PCD)-1 as a novel anti-nociceptive agent

被引：43

作者：

Chen, Wu-Fu ^{[1
,2
,3
]}

Huang, Shi-Ying ^{[4
]}

Liao, Chang-Yi ^{[5
]}

Sung, Chun-Sung ^{[6
,7
]}

Chen, Jyh-Yih ^{[8
]}

Wen, Zhi-Hong ^{[5
,9
,10
]}

机构：

[1] Kaohsiung Chang Gung Mem Hosp, Dept Neurosurg, Kaohsiung 833, Taiwan

[2] Chang Gung Univ, Coll Med, Kaohsiung 833, Taiwan

[3] Kaohsiung Chang Gung Mem Hosp, Ctr Parkinsons Dis, Kaohsiung 833, Taiwan

[4] Natl Sun Yat Sen Univ, Ctr Neurosci, Kaohsiung 804, Taiwan

[5] Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung 804, Taiwan

[6] Taipei Vet Gen Hosp, Dept Anesthesiol, Taipei 112, Taiwan

[7] Natl Yang Ming Univ, Sch Med, Taipei 112, Taiwan

[8] Acad Sinica, Inst Cellular & Organism Biol, Marine Res Stn, Jiaushi 262, Ilan, Taiwan

[9] Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Marine Biomed Lab, Kaohsiung 804, Taiwan

[10] Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Ctr Translat Biopharmaceut, Kaohsiung 804, Taiwan

来源：

BIOMATERIALS | 2015年 / 53卷

关键词：

Piscidin-1; Antimicrobial peptide; Chronic constriction injury; Neuropathic pain; Allodynia; Hyperalgesia; NITRIC-OXIDE SYNTHASE; HYBRID STRIPED BASS; ATTENUATES NOCICEPTIVE RESPONSES; CHRONIC CONSTRICTION INJURY; PERIPHERAL-NERVE INJURY; FORMOSAN SOFT CORAL; NEUROPATHIC PAIN; TRANSFORMING GROWTH-FACTOR-BETA-1; SPINAL-CORD; RAT MODEL;

D O I：

10.1016/j.biomaterials.2015.02.069

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The antimicrobial peptide piscidin (PCD)-1 has been reported to have antibacterial and immunomodulatory functions. Here, we investigated the anti-neuropathic properties of PCD-1, in order to determine its potential as a compound to alleviate pain. Treatment with PCD-1 suppressed the inflammatory proteins COX-2 and iNOS in murine macrophage (RAW264.7) and microglial (BV2) cell lines stimulated by lipopolysaccharide (LPS). For studies of the effect of PCD-1 in vivo, mononeuropathy in rats was induced by chronic constriction injury (CCI), and the resulting anti-nociceptive behaviors were compared between CCI controls and CCI rats given intrathecal injections of PCD-1. Much like gabapentin, PCD-1 exerts anti-nociceptive effects against thermal hyperalgesia, with a median effective dose (ED50) of 9.5 mu g in CCI rats. In CCI rats, PCD-1 exerted effects against mechanical and cold allodynia, thermal hyperalgesia, and weight-bearing deficits. Furthermore, CC-mediated activation of microglia and astrocytes in the dorsal horn of the lumbar spinal cord were decreased by PCD-1. In addition, PCD-1 suppressed up-regulation of interleukin-1 beta (IL-1 beta) and phosphorylated mammalian target of rapamycin (phospho-mTOR) in CCI rats. Finally, CU-induced down-regulation of transforming growth factor-beta 1 (TGF-beta 1) in rats was attenuated by injection of PCD-1. Taken together, the present findings demonstrate that the marine antimicrobial peptide PCD-1 has anti-nociceptive effects, and thus may have potential for development as an alternative pain-alleviating agent. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1 / 11

页数：11

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